caa a22 4500 44581747X CHVBK 20180317145233.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10815-010-9529-x doi (NATIONALLICENCE)springer-10.1007/s10815-010-9529-x Antioxidants rescue stressed embryos at a rate comparable with co-culturing of embryos with human umbilical cord mesenchymal cells [Elektronische Daten] [Ghazaleh Moshkdanian, Seyed Nematollahi-mahani, Fatemeh Pouya, Amirmahdi Nematollahi-mahani] Purpose: During laboratory manipulations, oocytes and embryos are inevitably exposed to suboptimal conditions that interfere with the normal development of embryos. Materials and methods: In this study, we examined the effects of antioxidants, feeder cells and a conditioned medium on embryo development and cleavage rate following exposure of the embryos to suboptimal conditions. We exposed mouse two-cell embryos to visible light and divided them into four groups: control (E-ctr), co-culture (Co-c), conditioned medium (Cndm) and antioxidant-plus medium (Aopm). We used human umbilical cord matrix-derived mesenchymal cells for co-culture. A group of embryos was not exposed to visible light and served as the non-exposed control (NE-ctr) group. Results: The developmental rate was higher in NE-ctr embryos than in the E-ctr group. Exposed embryos in the various groups showed a comparable developmental rate at different stages. Blastomere number significantly increased (P < 0.05) in the Co-c and Aopm groups compared with the E-ctr and Cndm groups. No significant difference was observed between the Co-c and Aopm groups. Conclusions: Our data indicate that in suboptimal conditions, antioxidants could improve the embryo cleavage rate in the same way as feeder cells. Antioxidants probably improve embryo quality through their ability to scavenge reactive oxygen species. Springer Science+Business Media, LLC, 2010 Visible light nationallicence Mouse embryos nationallicence Conditioned media nationallicence Antioxidants nationallicence Co-culture nationallicence Moshkdanian Ghazaleh Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Science, Kerman, Iran aut Nematollahi-mahani Seyed Kerman Neuroscience Research Center, Kerman University of Medical Science, Tahmasb-abad cross, Aviceena St, Kerman, Iran aut Pouya Fatemeh Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Science, Kerman, Iran aut Nematollahi-mahani Amirmahdi Afzal Research Institute, Kerman, Iran aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/4(2011-04-01), 343-349 1058-0468 28:4<343 2011 28 10815 https://doi.org/10.1007/s10815-010-9529-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9529-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moshkdanian Ghazaleh Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Science, Kerman, Iran aut NATIONALLICENCE 700 1- Nematollahi-mahani Seyed Kerman Neuroscience Research Center, Kerman University of Medical Science, Tahmasb-abad cross, Aviceena St, Kerman, Iran aut NATIONALLICENCE 700 1- Pouya Fatemeh Department of Anatomy, Afzalipour School of Medicine, Kerman University of Medical Science, Kerman, Iran aut NATIONALLICENCE 700 1- Nematollahi-mahani Amirmahdi Afzal Research Institute, Kerman, Iran aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/4(2011-04-01), 343-349 1058-0468 28:4<343 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer