caa a22 4500 445817496 CHVBK 20180317145233.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10815-010-9525-1 doi (NATIONALLICENCE)springer-10.1007/s10815-010-9525-1 Pressure induced nucleus DNA fragmentation [Elektronische Daten] [Cezary Grygoruk, Piotr Sieczynski, Jacek Modlinski, Barbara Gajda, Pawel Greda, Izabela Grad, Piotr Pietrewicz, Grzegorz Mrugacz] Purpose: The present study was designed to investigate the impact of pressure on nuclear DNA integrity in viable cells of mouse blastocysts. Methods: The blastocysts of hybrid F1 females [(C57Bl/10 J × CBA-H);N = 15] aged 2-3months were exposed into the pressure impulse lasting ~0.021s and characterized by a positive pressure peak of ~76mmHg. The nuclear DNA fragmentation index of mouse blastocysts was assessed by TUNEL assay within 60s after exposure to pressure impulse. Results: The mean nuclear DNA fragmentation index was significantly higher in the experimental group (83%) than in the control group (19.7%); p < 0.001. Conclusion(s): A low magnitude pressure impulse can induce nuclear DNA fragmentation in mouse blastocysts. The compression and decompression forces appearing during pressure fluctuations are responsible for the observed DNA shearing. Springer Science+Business Media, LLC, 2011 Embryo cell apoptosis nationallicence Pressure impulse nationallicence Pressure induced DNA fragmentation nationallicence Pressure DNA shearing nationallicence PDS nationallicence Grygoruk Cezary Center for Reproductive Medicine Bocian, Akademicka 26, 15-267, Bialystok, Poland aut Sieczynski Piotr Center for Reproductive Medicine Kriobank, Stoleczna 11, 15-879, Bialystok, Poland aut Modlinski Jacek Department of Experimental Embryology, Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Jastrzebiec, Postepu 1, 05-552, Wolka Kosowska, Poland aut Gajda Barbara Department of Biotechnology of Animal Reproduction, National Research Institute of Animal Production, Krakowska 1, 32-083, Balice/Krakow, Poland aut Greda Pawel Department of Experimental Embryology, Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Jastrzebiec, Postepu 1, 05-552, Wolka Kosowska, Poland aut Grad Izabela Department of Biotechnology of Animal Reproduction, National Research Institute of Animal Production, Krakowska 1, 32-083, Balice/Krakow, Poland aut Pietrewicz Piotr Center for Reproductive Medicine Bocian, Akademicka 26, 15-267, Bialystok, Poland aut Mrugacz Grzegorz Center for Reproductive Medicine Bocian, Akademicka 26, 15-267, Bialystok, Poland aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/4(2011-04-01), 363-368 1058-0468 28:4<363 2011 28 10815 https://doi.org/10.1007/s10815-010-9525-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9525-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Grygoruk Cezary Center for Reproductive Medicine Bocian, Akademicka 26, 15-267, Bialystok, Poland aut NATIONALLICENCE 700 1- Sieczynski Piotr Center for Reproductive Medicine Kriobank, Stoleczna 11, 15-879, Bialystok, Poland aut NATIONALLICENCE 700 1- Modlinski Jacek Department of Experimental Embryology, Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Jastrzebiec, Postepu 1, 05-552, Wolka Kosowska, Poland aut NATIONALLICENCE 700 1- Gajda Barbara Department of Biotechnology of Animal Reproduction, National Research Institute of Animal Production, Krakowska 1, 32-083, Balice/Krakow, Poland aut NATIONALLICENCE 700 1- Greda Pawel Department of Experimental Embryology, Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, Jastrzebiec, Postepu 1, 05-552, Wolka Kosowska, Poland aut NATIONALLICENCE 700 1- Grad Izabela Department of Biotechnology of Animal Reproduction, National Research Institute of Animal Production, Krakowska 1, 32-083, Balice/Krakow, Poland aut NATIONALLICENCE 700 1- Pietrewicz Piotr Center for Reproductive Medicine Bocian, Akademicka 26, 15-267, Bialystok, Poland aut NATIONALLICENCE 700 1- Mrugacz Grzegorz Center for Reproductive Medicine Bocian, Akademicka 26, 15-267, Bialystok, Poland aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/4(2011-04-01), 363-368 1058-0468 28:4<363 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer