caa a22 4500 44581750X CHVBK 20180317145233.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10815-010-9524-2 doi (NATIONALLICENCE)springer-10.1007/s10815-010-9524-2 Dynamic expression patterns of imprinted genes in human embryonic stem cells following prolonged passaging and differentiation [Elektronische Daten] [Xiuyun Mai, Qingyun Mai, Tao Li, Canquan Zhou] Purpose: To evaluate the overall expression patterns of imprinted genes in human embryonic stem cells following long term culture and differentiation. Materials and methods: Expression levels of 65 imprinted genes determined by PCR array were analyzed in one human embryonic stem cell line (cHES1) following prolonged passaging and differentiation. Results: Transcripts of 63 imprinted genes were detected in cHES1 cells. Expression levels of all but 5 imprinted genes did not correlate with passage numbers or differ in cells after passage 50 compared with those before passage 50. SLC22A2, SLC22A3, CPA, H19, COPG2IT1 and IGF2 expression were significantly increased in embryoid bodies compared with undifferentiated cells. Conclusions: The global expression profiles of imprinted genes are generally stable in human embryonic stem cells after prolonged passaging and differentiation. Springer Science+Business Media, LLC, 2010 Embryoid bodies nationallicence Epigenetic nationallicence Human embryonic stem cells nationallicence Imprinted gene nationallicence Mai Xiuyun Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan 2nd Avenue, Yuexiu, 510080, Guangzhou, China aut Mai Qingyun Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan 2nd Avenue, Yuexiu, 510080, Guangzhou, China aut Li Tao Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan 2nd Avenue, Yuexiu, 510080, Guangzhou, China aut Zhou Canquan Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan 2nd Avenue, Yuexiu, 510080, Guangzhou, China aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/4(2011-04-01), 315-323 1058-0468 28:4<315 2011 28 10815 https://doi.org/10.1007/s10815-010-9524-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9524-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mai Xiuyun Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan 2nd Avenue, Yuexiu, 510080, Guangzhou, China aut NATIONALLICENCE 700 1- Mai Qingyun Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan 2nd Avenue, Yuexiu, 510080, Guangzhou, China aut NATIONALLICENCE 700 1- Li Tao Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan 2nd Avenue, Yuexiu, 510080, Guangzhou, China aut NATIONALLICENCE 700 1- Zhou Canquan Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan 2nd Avenue, Yuexiu, 510080, Guangzhou, China aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/4(2011-04-01), 315-323 1058-0468 28:4<315 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer