caa a22 4500 445817534 CHVBK 20180317145233.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10815-010-9509-1 doi (NATIONALLICENCE)springer-10.1007/s10815-010-9509-1 DNA synthesis and epigenetic modification during mouse oocyte fertilization by human or hamster sperm injection [Elektronische Daten] [Yong-Nan Xu, Xiang-Shun Cui, Jin-Cheol Tae, Yong-Xun Jin, Nam-Hyung Kim] Purpose: To evaluate DNA synthesis and epigenetic modification in mouse oocytes during the first cell cycle following the injection of human or hamster sperm. Methods: Mouse oocytes following the injection of human and hamster sperm and cultured in M16 medium. Results: Male and female pronucleus formation, DNA synthesis, histone protein modification, and heterochromatin formation were similar in mouse oocytes injected with human or hamster sperm. However, DNA methylation patterns were altered in mouse oocytes following human sperm injection. Immunocytochemical staining with a histone H3-MeK9 antibody revealed that human and hamster sperm chromatin associated normally with female mouse chromatin, then entered into the metaphase and formed normal, two-cell stage embryos. Conclusions: Although differences in epigenetic modification of DNA were observed, fertilization and cleavage occurred in a species non-specific manner in mouse oocytes. Springer Science+Business Media, LLC, 2010 Interspecies ICSI nationallicence DNA synthesis nationallicence Epigenetic modification nationallicence Mouse oocyte nationallicence Xu Yong-Nan Department of Animal Sciences, Chungbuk National University, Gaesin-dong, 361-763, Cheongju, Chungbuk, South Korea aut Cui Xiang-Shun College of Laboratory Animal, Jilin University, 130062, Changchun, Jilin Province, China aut Tae Jin-Cheol Department of Animal Sciences, Chungbuk National University, Gaesin-dong, 361-763, Cheongju, Chungbuk, South Korea aut Jin Yong-Xun Department of Animal Sciences, Chungbuk National University, Gaesin-dong, 361-763, Cheongju, Chungbuk, South Korea aut Kim Nam-Hyung Department of Animal Sciences, Chungbuk National University, Gaesin-dong, 361-763, Cheongju, Chungbuk, South Korea aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/4(2011-04-01), 325-333 1058-0468 28:4<325 2011 28 10815 https://doi.org/10.1007/s10815-010-9509-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9509-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Yong-Nan Department of Animal Sciences, Chungbuk National University, Gaesin-dong, 361-763, Cheongju, Chungbuk, South Korea aut NATIONALLICENCE 700 1- Cui Xiang-Shun College of Laboratory Animal, Jilin University, 130062, Changchun, Jilin Province, China aut NATIONALLICENCE 700 1- Tae Jin-Cheol Department of Animal Sciences, Chungbuk National University, Gaesin-dong, 361-763, Cheongju, Chungbuk, South Korea aut NATIONALLICENCE 700 1- Jin Yong-Xun Department of Animal Sciences, Chungbuk National University, Gaesin-dong, 361-763, Cheongju, Chungbuk, South Korea aut NATIONALLICENCE 700 1- Kim Nam-Hyung Department of Animal Sciences, Chungbuk National University, Gaesin-dong, 361-763, Cheongju, Chungbuk, South Korea aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/4(2011-04-01), 325-333 1058-0468 28:4<325 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer