caa a22 4500 445817550 CHVBK 20180317145233.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10815-010-9522-4 doi (NATIONALLICENCE)springer-10.1007/s10815-010-9522-4 In vitro and in vivo viability of human blastocysts collapsed by laser pulse or osmotic shock prior to vitrification [Elektronische Daten] [Hiroshi Iwayama, Shinichi Hochi, Masanori Yamashita] Purpose: This study was designed to investigate whether artificial shrinkage, induced by a laser pulse or hyperosmotic sucrose solutions, improves in vitro survival and/or implantation of vitrified-warmed human expanded blastocysts. Methods: Before Cryotop vitrification, the blastocoelic cavity was collapsed either by a laser pulse or sucrose solutions. Non-treated blastocysts were used as control. Post-warm blastocyst survival and implantation after transfer were examined. Implantation rate outcome was retrospectively analyzed by morphological grading and developmental kinetics of post-warm blastocysts. Results: Survival rates in the three groups were high. Implantation rates in the laser-pulse group (59.7%) were comparable with those in the sucrose group (49.3%), and were significantly higher than those in the control group (34.2%). The proportion of blastocysts showing fast development tended to be higher when the blastocysts underwent artificial shrinkage treatment before vitrification. There was no clear correlation between morphology of post-warm blastocysts and implantation rate. Conclusion: Artificial shrinkage treatment before vitrification is associated with an increased probability of fast-developing embryos, resulting in higher implantation rates. Springer Science+Business Media, LLC, 2010 Artificial shrinkage nationallicence Clinical pregnancy nationallicence Laser pulse nationallicence Sucrose nationallicence Vitrification nationallicence Iwayama Hiroshi Yamashita Ladies' Clinic, 651-0086, Kobe, Hyogo, Japan aut Hochi Shinichi Faculty of Textile Science and Technology, Shinshu University, 386-8567, Ueda, Nagano, Japan aut Yamashita Masanori Yamashita Ladies' Clinic, 651-0086, Kobe, Hyogo, Japan aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/4(2011-04-01), 355-361 1058-0468 28:4<355 2011 28 10815 https://doi.org/10.1007/s10815-010-9522-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9522-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Iwayama Hiroshi Yamashita Ladies' Clinic, 651-0086, Kobe, Hyogo, Japan aut NATIONALLICENCE 700 1- Hochi Shinichi Faculty of Textile Science and Technology, Shinshu University, 386-8567, Ueda, Nagano, Japan aut NATIONALLICENCE 700 1- Yamashita Masanori Yamashita Ladies' Clinic, 651-0086, Kobe, Hyogo, Japan aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/4(2011-04-01), 355-361 1058-0468 28:4<355 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer