caa a22 4500 445817577 CHVBK 20180317145233.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s10815-010-9517-1 doi (NATIONALLICENCE)springer-10.1007/s10815-010-9517-1 Genetic and epigenetic X-chromosome variations in a parthenogenetic human embryonic stem cell line [Elektronische Daten] [Weiqiang Liu, Yifei Yin, Yonghua Jiang, Chaohui Kou, Yumei Luo, Shengchang Huang, Yuhong Zheng, Shaoying Li, Qing Li, Liyuan Guo, Shaorong Gao, Xiaofang Sun] Purpose: To assess the genetic and epigenetic status of parthenogenetic human embryonic stem cells (phESCs). Methods: Cytogenetics, X chromosome inactivation (XCI) and gene expression patterns were analyzed in one phESC line (FY-phES-018) that was derived from our laboratory. Results: FY-phES-018 cells displayed the classical characteristics of normal hESCs. These cells had a 46, XX karyotype, and no inactive X chromosomes were observed before passage 20. After being cultured long term in vitro, some cells lost one X, and the proportion of cells with only one X gradually increased. At passage 35, almost all the cells displayed a 45, XO karyotype. Interestingly, at passage 45, the recovery of the X-chromosome was observed, and XCI became detectable; the mosaic ratio of 46, XX to 45, XO was 67:33. After passage 60, most cells displayed the 46, XX karyotype again with a mosaic ratio of 97:3. Some aberrant genomic imprinting was also observed in these cells. Conclusions: The phESCs line FY-phES-018 is both genetically and epigenetically unstable; therefore, further research is needed before using these cells. Springer Science+Business Media, LLC, 2010 Parthenogenetic nationallicence hESC nationallicence Genetic nationallicence Epigenetic nationallicence Instability nationallicence Liu Weiqiang Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut Yin Yifei Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut Jiang Yonghua Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut Kou Chaohui National Institute of Biological Sciences (NIBS), 102206, Beijing, China aut Luo Yumei Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut Huang Shengchang Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut Zheng Yuhong Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut Li Shaoying Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut Li Qing Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut Guo Liyuan Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut Gao Shaorong National Institute of Biological Sciences (NIBS), 102206, Beijing, China aut Sun Xiaofang Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/4(2011-04-01), 303-313 1058-0468 28:4<303 2011 28 10815 https://doi.org/10.1007/s10815-010-9517-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9517-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Weiqiang Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut NATIONALLICENCE 700 1- Yin Yifei Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut NATIONALLICENCE 700 1- Jiang Yonghua Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut NATIONALLICENCE 700 1- Kou Chaohui National Institute of Biological Sciences (NIBS), 102206, Beijing, China aut NATIONALLICENCE 700 1- Luo Yumei Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut NATIONALLICENCE 700 1- Huang Shengchang Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut NATIONALLICENCE 700 1- Zheng Yuhong Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut NATIONALLICENCE 700 1- Li Shaoying Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut NATIONALLICENCE 700 1- Li Qing Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut NATIONALLICENCE 700 1- Guo Liyuan Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut NATIONALLICENCE 700 1- Gao Shaorong National Institute of Biological Sciences (NIBS), 102206, Beijing, China aut NATIONALLICENCE 700 1- Sun Xiaofang Guangzhou Key Laboratory of Reproductive and Genetics; Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical College, 510150, Guangzhou, China aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/4(2011-04-01), 303-313 1058-0468 28:4<303 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer