caa a22 4500 445817615 CHVBK 20180317145233.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10815-011-9574-0 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9574-0 Study of GT-repeat expansion in Heme oxygenase-1 gene promoter as genetic cause of male infertility [Elektronische Daten] [Elham Siasi, Ahmad Aleyasin, Seyed Mowla, Hamid Sahebkashaf] Purpose: The length of GT-repeats polymorphic region in the promoter of human Heme oxygenase-1 gene (HO-1) alters the level of its transcriptional activity in response to oxidative stresses. Decreased level of HO-1 protein in the seminal plasma has been reported to be associated with oligospermia and azoospermia in male infertility. This is the first study to investigate the association between GT-repeats expansion in the promoter of the HO-1 gene and male infertility. Methods: The frequencies of different GT-repeats alleles in the promoter of HO-1 gene were determined in 100 cases and 100 normal controls using PCR-PAGE, ABI fragment analysis genotyping and sequencing analysis. Results: All alleles were classified into S and L alleles. S alleles were specified as number 0 to 3 with <27 GT-repeats and L alleles were specified as number 4 to 6 with >27 repeats. The L allele frequency was significantly higher among case group (54.5%) than that was obtained in the normal control group (37.5%). Statistical analysis provided a significant relationship between L allele and male infertility (P < 0.001). Conclusions: This study shows for the first time that GT-repeats expansion in promoter of the HO-1 gene is associated with oligospermia and azoospermia among Iranian infertile cases. Springer Science+Business Media, LLC, 2011 Male infertility nationallicence Heme oxygenase-1 gene nationallicence Polymorphism nationallicence Oxidative stress nationallicence Siasi Elham Tarbiat Modarres University, Tehran, Iran aut Aleyasin Ahmad National Institute of Genetic Engineering and Biotechnology, Tehran, Iran aut Mowla Seyed Tarbiat Modarres University, Tehran, Iran aut Sahebkashaf Hamid Navide Institute of Infertility, Tehran, Iran aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/8(2011-08-01), 737-741 1058-0468 28:8<737 2011 28 10815 https://doi.org/10.1007/s10815-011-9574-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9574-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Siasi Elham Tarbiat Modarres University, Tehran, Iran aut NATIONALLICENCE 700 1- Aleyasin Ahmad National Institute of Genetic Engineering and Biotechnology, Tehran, Iran aut NATIONALLICENCE 700 1- Mowla Seyed Tarbiat Modarres University, Tehran, Iran aut NATIONALLICENCE 700 1- Sahebkashaf Hamid Navide Institute of Infertility, Tehran, Iran aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/8(2011-08-01), 737-741 1058-0468 28:8<737 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer