caa a22 4500 445817658 CHVBK 20180317145233.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10815-011-9584-y doi (NATIONALLICENCE)springer-10.1007/s10815-011-9584-y Digital holographic microscopy in human sperm imaging [Elektronische Daten] [Igor Crha, Jana Zakova, Martin Huser, Pavel Ventruba, Eva Lousova, Michal Pohanka] Purpose: The aim of this study was to use digital holographic microscopy (DHM) in human sperm imaging and compare quantitative phase contrast of sperm heads in normozoospermia (NZ) and oligoasthenozoospermia (OAT). Methods: DHM spermatozoa imaging and repeated quantitative phase shift evaluation were used. Five NZ and 5 OAT samples were examined. Semen samples were examined by semen analysis and processed for DHM. Main outcome measures were maximum phase shift value of the sperm heads. Differences of the phase shift and in NZ and OAT samples were statistically tested. Results: In NZ samples median phase shifts were in the range 2.72-3.21rad and 2.00-2.15 in OAT samples. Differences among individual samples were statistically significant (p < 0.001) in both groups. Median phase shift according to sperm count was 2.90rad in NZ samples and 2.00rad in OAT samples. This difference was statistically significant (p < 0.001). Conclusion: Quantitative evaluation of the phase shift by DHM could provide new information on the exact structure and composition of the sperm head. At present, this technique is not established for clinical utility. Springer Science+Business Media, LLC, 2011 Digital holographic microscopy nationallicence Sperm imaging nationallicence Spermatozoon nationallicence Male infertility nationallicence Chromatin integrity nationallicence Crha Igor Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University, and Faculty Hospital, Obilni trh 11, 602 00, Brno, Czech Republic aut Zakova Jana Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University, and Faculty Hospital, Obilni trh 11, 602 00, Brno, Czech Republic aut Huser Martin Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University, and Faculty Hospital, Obilni trh 11, 602 00, Brno, Czech Republic aut Ventruba Pavel Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University, and Faculty Hospital, Obilni trh 11, 602 00, Brno, Czech Republic aut Lousova Eva Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University, and Faculty Hospital, Obilni trh 11, 602 00, Brno, Czech Republic aut Pohanka Michal Department of Functional Diagnostics and Rehabilitation, St. Anne's Faculty Hospital, Brno, Czech Republic aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/8(2011-08-01), 725-729 1058-0468 28:8<725 2011 28 10815 https://doi.org/10.1007/s10815-011-9584-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9584-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Crha Igor Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University, and Faculty Hospital, Obilni trh 11, 602 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Zakova Jana Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University, and Faculty Hospital, Obilni trh 11, 602 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Huser Martin Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University, and Faculty Hospital, Obilni trh 11, 602 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Ventruba Pavel Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University, and Faculty Hospital, Obilni trh 11, 602 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Lousova Eva Department of Gynecology and Obstetrics, Faculty of Medicine, Masaryk University, and Faculty Hospital, Obilni trh 11, 602 00, Brno, Czech Republic aut NATIONALLICENCE 700 1- Pohanka Michal Department of Functional Diagnostics and Rehabilitation, St. Anne's Faculty Hospital, Brno, Czech Republic aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/8(2011-08-01), 725-729 1058-0468 28:8<725 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer