caa a22 4500 445817690 CHVBK 20180317145233.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10815-011-9587-8 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9587-8 A potential use of embryonic stem cell medium for the in vitro culture of preimplantation embryos [Elektronische Daten] [Katherine Gelber, Aileen Tamura, Vernadeth Alarcon, Yusuke Marikawa] Purpose: To assess the impact of embryonic stem cell culture medium (ESCM) on the pre- and post-implantation development of the mouse embryo, as a mammalian model, in comparison with the conventional culture medium, a potassium simplex optimized medium (KSOM). Methods: Development in ESCM versus KSOM was compared in terms of embryo morphology, cleavage, cavitation, hatching, cell number, expression of TE and ICM transcription factors (Cdx2 and Oct4, respectively), implantation, and development in utero. Results: An enriched medium like ESCM can be beneficial for in vitro embryo development when cultured from the 8-cell stage, as evidenced by promotion of blastocyst development with respect to cavity expansion, hatching, and cell division. Such benefits were not observed when embryos were cultured from the 2-cell stage. Conclusions: ESCM may augment in vitro embryo development from the 8-cell stage. Using different culture media at different stages may be beneficial to achieve more effective human in vitro fertilization. Springer Science+Business Media, LLC, 2011 Assisted reproductive technologies (ART) nationallicence Culture media nationallicence Embryonic stem cell medium (ESCM) nationallicence Potassium simplex optimized medium (KSOM) nationallicence Preimplantation development nationallicence Gelber Katherine Institute for Biogenesis Research, Department of Anatomy, Biochemistry and Physiology, University of Hawaii John A. Burns School of Medicine, 651 Ilalo St., Biosciences Building, 96813, Honolulu, HI, USA aut Tamura Aileen Institute for Biogenesis Research, Department of Anatomy, Biochemistry and Physiology, University of Hawaii John A. Burns School of Medicine, 651 Ilalo St., Biosciences Building, 96813, Honolulu, HI, USA aut Alarcon Vernadeth Institute for Biogenesis Research, Department of Anatomy, Biochemistry and Physiology, University of Hawaii John A. Burns School of Medicine, 651 Ilalo St., Biosciences Building, 96813, Honolulu, HI, USA aut Marikawa Yusuke Institute for Biogenesis Research, Department of Anatomy, Biochemistry and Physiology, University of Hawaii John A. Burns School of Medicine, 651 Ilalo St., Biosciences Building, 96813, Honolulu, HI, USA aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/8(2011-08-01), 659-668 1058-0468 28:8<659 2011 28 10815 https://doi.org/10.1007/s10815-011-9587-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9587-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gelber Katherine Institute for Biogenesis Research, Department of Anatomy, Biochemistry and Physiology, University of Hawaii John A. Burns School of Medicine, 651 Ilalo St., Biosciences Building, 96813, Honolulu, HI, USA aut NATIONALLICENCE 700 1- Tamura Aileen Institute for Biogenesis Research, Department of Anatomy, Biochemistry and Physiology, University of Hawaii John A. Burns School of Medicine, 651 Ilalo St., Biosciences Building, 96813, Honolulu, HI, USA aut NATIONALLICENCE 700 1- Alarcon Vernadeth Institute for Biogenesis Research, Department of Anatomy, Biochemistry and Physiology, University of Hawaii John A. Burns School of Medicine, 651 Ilalo St., Biosciences Building, 96813, Honolulu, HI, USA aut NATIONALLICENCE 700 1- Marikawa Yusuke Institute for Biogenesis Research, Department of Anatomy, Biochemistry and Physiology, University of Hawaii John A. Burns School of Medicine, 651 Ilalo St., Biosciences Building, 96813, Honolulu, HI, USA aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/8(2011-08-01), 659-668 1058-0468 28:8<659 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer