caa a22 4500 445817712 CHVBK 20180317145234.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10815-011-9571-3 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9571-3 An association study of SPO11 gene single nucleotide polymorphisms with idiopathic male infertility in Chinese Han population [Elektronische Daten] [Jing Zhang, Dang-xia Zhou, Hai-xu Wang, Zhao Tian] Purpose: To investigate the incidence of single nucleotide polymorphisms in SPO11 and its influence in idiopathic male infertility in China. Methods: Infertility factors such as anatomical, immunological and infectious disorders were examined in selecting patients with idiopathic male infertility. Routine semen analysis was performed. DNA was isolated from peripheral blood of the selected patients and control group, and five SNP loci of SPO11 were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Furthermore, nucleotide sequences were sequenced. Results: SNP5 (rs28368082) in the exon7 of SPO11 was identified to be associated with idiopathic male infertility (P = 0.037 for differences across genotypes). A transversion (C5679T) was detected in eight patients (11.0%), which led arginine change into tryptophan. And this variant was not found in the remaining patients and controls. Conclusion: A SPO11 SNP was associated with idiopathic male reproduction, suggested that SPO11 might has an effect on premorbid functioning, which increase susceptibility for idiopathic male reproduction. Further research on this issue is still necessary. Springer Science+Business Media, LLC, 2011 Idiopathic male infertility nationallicence Single nucleotide polymorphisms (SNP) nationallicence Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) nationallicence SPO11 nationallicence Zhang Jing Department of Pathology, Medical School, Xi'an Jiaotong University, 710061, Xi'an, China aut Zhou Dang-xia Department of Pathology, Medical School, Xi'an Jiaotong University, 710061, Xi'an, China aut Wang Hai-xu Reproductive Medical Center, Xi'an Jiaotong University, 710061, Xi'an, China aut Tian Zhao Department of Forensic Science, Medical School, Xi'an Jiaotong University, 710061, Xi'an, China aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/8(2011-08-01), 731-736 1058-0468 28:8<731 2011 28 10815 https://doi.org/10.1007/s10815-011-9571-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9571-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Jing Department of Pathology, Medical School, Xi'an Jiaotong University, 710061, Xi'an, China aut NATIONALLICENCE 700 1- Zhou Dang-xia Department of Pathology, Medical School, Xi'an Jiaotong University, 710061, Xi'an, China aut NATIONALLICENCE 700 1- Wang Hai-xu Reproductive Medical Center, Xi'an Jiaotong University, 710061, Xi'an, China aut NATIONALLICENCE 700 1- Tian Zhao Department of Forensic Science, Medical School, Xi'an Jiaotong University, 710061, Xi'an, China aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/8(2011-08-01), 731-736 1058-0468 28:8<731 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer