caa a22 4500 445817720 CHVBK 20180317145234.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10815-011-9572-2 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9572-2 Derivation of buffalo embryonic stem-like cells from in vitro-produced blastocysts on homologous and heterologous feeder cells [Elektronische Daten] [Dharmendra Kumar, T. Anand, K. Singh, M. Singh, R. Shah, M. Chauhan, P. Palta, S. Singla, R. Manik] Purpose: The aim of the present study is to compare the ability of homologous and heterologous embryonic fibroblast feeder layers to support isolation and proliferation of buffalo ES-like cells generated from hatched and expanded blastocysts produced by in vitro fertilization and characterization of derived cells through expression of pluripotent markers. Methods: Embryonic stem cells were derived from hatched and expanded blastocysts through intact blastocyst culture and enzymatic method respectively and compared for proliferation rate on homologous (buffalo) and heterologous feeder layers (goat and sheep). Results: A total of 69 hatched and 83 expanded blastocysts were used for isolation of inner cell masses which were seeded on buffalo, goat and sheep embryonic feeder layers. Following seeding, attachment rate, primary colony formation rate and survival to maximum number of passages were observed to be higher on homologous feeder layers. Conclusions: Upon comparison of different feeder layer cells for derivation and maintenance of buffalo ES-like cells from hatched and expanded blastocysts, buffalo embryonic fibroblast cells were able to provide a better environment for maintaining pluripotency in culture conditions. Springer Science+Business Media, LLC, 2011 Buffalo nationallicence Characterization nationallicence Fibroblast nationallicence NANOG nationallicence OCT-4 nationallicence Kumar Dharmendra Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut Anand T. Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut Singh K. Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut Singh M. Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut Shah R. Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut Chauhan M. Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut Palta P. Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut Singla S. Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut Manik R. Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/8(2011-08-01), 679-688 1058-0468 28:8<679 2011 28 10815 https://doi.org/10.1007/s10815-011-9572-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9572-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kumar Dharmendra Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut NATIONALLICENCE 700 1- Anand T. Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut NATIONALLICENCE 700 1- Singh K. Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut NATIONALLICENCE 700 1- Singh M. Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut NATIONALLICENCE 700 1- Shah R. Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut NATIONALLICENCE 700 1- Chauhan M. Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut NATIONALLICENCE 700 1- Palta P. Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut NATIONALLICENCE 700 1- Singla S. Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut NATIONALLICENCE 700 1- Manik R. Animal Biotechnology Centre, National Dairy Research Institute, 132001, Karnal, India aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/8(2011-08-01), 679-688 1058-0468 28:8<679 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer