caa a22 4500 445817739 CHVBK 20180317145234.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10815-011-9582-0 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9582-0 Biological pH buffers in IVF: help or hindrance to success [Elektronische Daten] [Matthew Will, Natalie Clark, Jason Swain] Purpose: Minimizing environmental stress helps maintain cellular homeostasis and is a crucial component in optimizing embryo development in vitro and resulting ART success. One stressor of particular interest is pH. Biologic buffers, such as HEPES and MOPS, are valuable tools for stabilizing pH. The objective of this manuscript is to summarize efficacy and impact of various pH buffers used during IVF lab procedures Methods: Keyword searches were performed using Pubmed and Medline and relevant literature reviewed. Results: Various pH buffers have been used with varying degrees of success for gamete and embryo processing in a variety of animal species, as well as in human. Conclusion: Though biologic buffers off a means to improve pH stability, not all buffers may be appropriate for use with gametes and embryos. Specific buffers may have undesired effects, and these may be buffer, species, cell type or concentration dependent. Continued research is needed to further refine and improve the use of biologic buffers for use in human ART. Springer Science+Business Media, LLC, 2011 pH nationallicence Zwitterion nationallicence Good's buffer nationallicence Gamete nationallicence Embryo nationallicence Will Matthew Department of Obstetrics & Gynecology, University of Michigan, 48108, Ann Arbor, MI, USA aut Clark Natalie Department of Obstetrics & Gynecology, University of Michigan, 48108, Ann Arbor, MI, USA aut Swain Jason Department of Obstetrics & Gynecology, University of Michigan, 48108, Ann Arbor, MI, USA aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/8(2011-08-01), 711-724 1058-0468 28:8<711 2011 28 10815 https://doi.org/10.1007/s10815-011-9582-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9582-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Will Matthew Department of Obstetrics & Gynecology, University of Michigan, 48108, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Clark Natalie Department of Obstetrics & Gynecology, University of Michigan, 48108, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Swain Jason Department of Obstetrics & Gynecology, University of Michigan, 48108, Ann Arbor, MI, USA aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/8(2011-08-01), 711-724 1058-0468 28:8<711 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer