caa a22 4500 445817798 CHVBK 20180317145234.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10815-011-9541-9 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9541-9 Sertoli cell-conditioned medium induces germ cell differentiation in human embryonic stem cells [Elektronische Daten] [Mieke Geens, Karen Sermon, Hilde Van de Velde, Herman Tournaye] Purpose: To investigate the spontaneous germ cell differentiation capacity of VUB hESC lines, develop a protocol for the induction of germ cell differentiation using conditioned medium from Sertoli cells (SCCM) and compare it to existing protocols. Methods: hESC were allowed to differentiate spontaneously or after the addition of bone morphogenetic proteins (BMPs) and/or SCCM. VASA transcripts were measured by relative quantification real-time RT-PCR to determine the efficiency of germ cell differentiation. Results: VUB hESC lines can differentiate spontaneously towards the germ cell lineage, however, more consistently in an embryoid body approach than in monolayer cultures. BMPs and SCCM significantly improve VASA expression, but do not have a synergistic effect. Direct contact of differentiating hESC with Sertoli cells does not improve VASA expression. Conclusions: SCCM contains inductive factors for germ cell differentiation and could represent an element for in-vitro differentiation to germ cells. Springer Science+Business Media, LLC, 2011 hESC nationallicence Sertoli cells nationallicence Germ cells nationallicence Differentiation nationallicence Embryoid bodies nationallicence Geens Mieke Research group Biology of the Testis (BITE), Department of Embryology and Genetics, Medical School of the Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut Sermon Karen Research group Reproduction and Genetics (REGE), Department of Embryology and Genetics, Medical School of the Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut Van de Velde Hilde Research group Reproduction and Genetics (REGE), Department of Embryology and Genetics, Medical School of the Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut Tournaye Herman Research group Biology of the Testis (BITE), Department of Embryology and Genetics, Medical School of the Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/5(2011-05-01), 471-480 1058-0468 28:5<471 2011 28 10815 https://doi.org/10.1007/s10815-011-9541-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9541-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Geens Mieke Research group Biology of the Testis (BITE), Department of Embryology and Genetics, Medical School of the Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut NATIONALLICENCE 700 1- Sermon Karen Research group Reproduction and Genetics (REGE), Department of Embryology and Genetics, Medical School of the Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut NATIONALLICENCE 700 1- Van de Velde Hilde Research group Reproduction and Genetics (REGE), Department of Embryology and Genetics, Medical School of the Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut NATIONALLICENCE 700 1- Tournaye Herman Research group Biology of the Testis (BITE), Department of Embryology and Genetics, Medical School of the Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/5(2011-05-01), 471-480 1058-0468 28:5<471 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer