caa a22 4500 445817836 CHVBK 20180317145234.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10815-011-9537-5 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9537-5 The effects of male aging on semen quality, sperm DNA fragmentation and chromosomal abnormalities in an infertile population [Elektronische Daten] [Sonia Brahem, Meriem Mehdi, Hatem Elghezal, Ali Saad] Purpose: To investigate the effects of male aging on semen quality, DNA fragmentation and chromosomal abnormalities in the spermatozoa of infertile patients and fertile men. Methods: Semen samples of 140 infertile patients (24-76years) and 50 men with proven fertility (25-65years) were analyzed according to WHO guidelines. DNA fragmentation was detected by TUNEL assay, while aneuploidy was assessed by FISH. Results: In the patient group, semen volume and vitality of spermatozoa decreased significantly with age, while sperm concentration showed a statistically significant increase with age. DNA fragmentation as well as disomy of sex chromosomes and disomy 8 did not show a statistically significant change with age. However, the diploidy rate was significantly increased with patient's age. In the control group, conventional semen parameters as well as DNA fragmentation and chromosomal abnormalities did not show a statistically significant with age. Conclusion: Increased age in infertile men is associated with an increase in sperm concentration and diploidy, as well as a decline in semen volume and sperm vitality. However motility, morphology and DNA fragmentation are not affected by male age. Springer Science+Business Media, LLC, 2011 Aneuploidy nationallicence DNA fragmentation nationallicence Male infertility nationallicence Paternal age nationallicence Semen parameters nationallicence Brahem Sonia Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut Mehdi Meriem Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut Elghezal Hatem Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut Saad Ali Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/5(2011-05-01), 425-432 1058-0468 28:5<425 2011 28 10815 https://doi.org/10.1007/s10815-011-9537-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9537-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Brahem Sonia Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut NATIONALLICENCE 700 1- Mehdi Meriem Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut NATIONALLICENCE 700 1- Elghezal Hatem Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut NATIONALLICENCE 700 1- Saad Ali Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000, Sousse, Tunisia aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/5(2011-05-01), 425-432 1058-0468 28:5<425 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer