caa a22 4500 445817852 CHVBK 20180317145234.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10815-011-9547-3 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9547-3 The human postmenopausal ovary as a tool for evaluation of cryopreservation protocols towards whole ovary cryopreservation [Elektronische Daten] [Milan Milenkovic, Manda Gharemani, Anette Bergh, Ann Wallin, Johan Mölne, Elvedin Fazlagic, Eirik Eliassen, Jarl Kahn, Mats Brännström] Purpose: Cryopreservation of a complete ovary may be a future method for fertility preservation in cancer patients. Difficulties exist in cryopreservation of the relatively large ovarian tissue mass. This study evaluates whether a human postmenopausal ovary can be used, as a complement to animal models, in studies of this research field. Methods: Postmenopausal human ovaries (n = 10) were isolated and flushed through ovarian arteries with either the cryoprotectant dimethylsulphoxide or Ringer-Acetate, followed by slow freezing. After thawing, production of androgens during in vitro perfusion and morphology (light/electron microscopy) were assessed. Results: The dimethylsulphoxide-cryopreserved ovaries showed larger secretion of androgens during perfusion than Ringer Acetate-cryopreserved ovaries. Light microscopy showed well preserved morphology in both groups. Electron microscopy revealed normal appearance of stroma and vessels in the dimethylsulphoxide group. Conclusions: The study demonstrates the potential to use the postmenopausal human ovary for further studies aiming at optimizing cryopreservation protocols, with special reference to ovarian vascularity and stroma. Springer Science+Business Media, LLC, 2011 Androgens nationallicence Cryopreservation nationallicence Morphology nationallicence Ovary nationallicence Perfusion nationallicence Milenkovic Milan Department of Obstetrics and Gynecology, Sahlgrenska Academy, University of Gothenburg, SE-41345, Gothenburg, Sweden aut Gharemani Manda Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, BC, Canada aut Bergh Anette Division of Obstetrics and Gynecology, Telemark Hospital, 3710, Skien, Norway aut Wallin Ann Department of Obstetrics and Gynecology, Sahlgrenska Academy, University of Gothenburg, SE-41345, Gothenburg, Sweden aut Mölne Johan Department of Clinical Pathology, Sahlgrenska Academy, University of Gothenburg, SE-41345, Gothenburg, Sweden aut Fazlagic Elvedin Division of Plastic Surgery, Telemark Hospital, 3710, Skien, Norway aut Eliassen Eirik Division of Obstetrics and Gynecology, Telemark Hospital, 3710, Skien, Norway aut Kahn Jarl Division of Obstetrics and Gynecology, Telemark Hospital, 3710, Skien, Norway aut Brännström Mats Department of Obstetrics and Gynecology, Sahlgrenska Academy, University of Gothenburg, SE-41345, Gothenburg, Sweden aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/5(2011-05-01), 453-460 1058-0468 28:5<453 2011 28 10815 https://doi.org/10.1007/s10815-011-9547-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9547-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Milenkovic Milan Department of Obstetrics and Gynecology, Sahlgrenska Academy, University of Gothenburg, SE-41345, Gothenburg, Sweden aut NATIONALLICENCE 700 1- Gharemani Manda Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, BC, Canada aut NATIONALLICENCE 700 1- Bergh Anette Division of Obstetrics and Gynecology, Telemark Hospital, 3710, Skien, Norway aut NATIONALLICENCE 700 1- Wallin Ann Department of Obstetrics and Gynecology, Sahlgrenska Academy, University of Gothenburg, SE-41345, Gothenburg, Sweden aut NATIONALLICENCE 700 1- Mölne Johan Department of Clinical Pathology, Sahlgrenska Academy, University of Gothenburg, SE-41345, Gothenburg, Sweden aut NATIONALLICENCE 700 1- Fazlagic Elvedin Division of Plastic Surgery, Telemark Hospital, 3710, Skien, Norway aut NATIONALLICENCE 700 1- Eliassen Eirik Division of Obstetrics and Gynecology, Telemark Hospital, 3710, Skien, Norway aut NATIONALLICENCE 700 1- Kahn Jarl Division of Obstetrics and Gynecology, Telemark Hospital, 3710, Skien, Norway aut NATIONALLICENCE 700 1- Brännström Mats Department of Obstetrics and Gynecology, Sahlgrenska Academy, University of Gothenburg, SE-41345, Gothenburg, Sweden aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/5(2011-05-01), 453-460 1058-0468 28:5<453 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer