caa a22 4500 445817860 CHVBK 20180317145234.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10815-011-9539-3 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9539-3 Cryopreservation of whole ovaries with vascular pedicles: vitrification or conventional freezing? [Elektronische Daten] [Jian-Min Zhang, Yan Sheng, Yong-Zhi Cao, Hong-Yan Wang, Zi-Jiang Chen] Purpose: To compare the efficacy of vitrification and conventional freezing of whole ovaries. Methods: Ovaries obtained from 5-year-old female bovines were cryopreserved by conventional freezing, rapid freezing and vitrification. The ovarian cortical strips were cryopreserved by conventional freezing. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles, hormones concentrations in the culture supernatants, and lactate dehyrogenase levels were measured. Results: The efficacy of cryopreservation of whole ovaries by vitrification was higher than those by conventional freezing and rapid freezing. Conventional freezing of ovarian cortical strips was more effective than cryopreservation of whole ovaries by conventional freezing, rapid freezing, and vitrification. Conclusions: Vitrification seems to be more suitable than conventional freezing for cryopreservation of whole ovaries. However, further studies are required to improve the efficacy of vitrifying whole ovaries. Springer Science+Business Media, LLC, 2011 Conventional freezing nationallicence Cryopreservation nationallicence Rapid freezing nationallicence Vitrification nationallicence Whole ovaries nationallicence Zhang Jian-Min Shandong Provincial Key Laboratory of Reproductive Medicine, Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, 250021, Jinan, China aut Sheng Yan Shandong Provincial Key Laboratory of Reproductive Medicine, Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, 250021, Jinan, China aut Cao Yong-Zhi Shandong Provincial Key Laboratory of Reproductive Medicine, Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, 250021, Jinan, China aut Wang Hong-Yan Shandong Provincial Key Laboratory of Reproductive Medicine, Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, 250021, Jinan, China aut Chen Zi-Jiang Shandong Provincial Key Laboratory of Reproductive Medicine, Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, 250021, Jinan, China aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/5(2011-05-01), 445-452 1058-0468 28:5<445 2011 28 10815 https://doi.org/10.1007/s10815-011-9539-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9539-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Jian-Min Shandong Provincial Key Laboratory of Reproductive Medicine, Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, 250021, Jinan, China aut NATIONALLICENCE 700 1- Sheng Yan Shandong Provincial Key Laboratory of Reproductive Medicine, Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, 250021, Jinan, China aut NATIONALLICENCE 700 1- Cao Yong-Zhi Shandong Provincial Key Laboratory of Reproductive Medicine, Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, 250021, Jinan, China aut NATIONALLICENCE 700 1- Wang Hong-Yan Shandong Provincial Key Laboratory of Reproductive Medicine, Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, 250021, Jinan, China aut NATIONALLICENCE 700 1- Chen Zi-Jiang Shandong Provincial Key Laboratory of Reproductive Medicine, Center for Reproductive Medicine, Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, 250021, Jinan, China aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/5(2011-05-01), 445-452 1058-0468 28:5<445 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer