caa a22 4500 445817909 CHVBK 20180317145234.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10815-010-9500-x doi (NATIONALLICENCE)springer-10.1007/s10815-010-9500-x Vitrification of mouse embryo-derived ICM cells: a tool for preserving embryonic stem cell potential? [Elektronische Daten] [Nina Desai, Jing Xu, Tamara Tsulaia, Julia Szeptycki-Lawson, Faten AbdelHafez, James Goldfarb, Tommaso Falcone] Purpose: Vitrification technology presents new opportunities for preservation of embryo derived stem cells without first establishing a viable ESC line. This study tests the feasibility of cryopreserving ICM cells using vitrification. Materials and Methods: ICMs from mouse embryos were isolated and vitrified in HSV straws or on cryoloops. Upon warming, the vitrified ICMs were cultured and observed for attachment and morphology. Colonies were passaged every 3-6days. ICMs and ICM-derived ESC colonies were tested for expression of stem cell specific markers. Results: ICMs vitrified on both the cryoloop and the HSV straw had high survival rates. ICM derived ESCs remained undifferentiated for several passages and demonstrated expression of typical stem cell markers; SSEA-1, Sox-2, Oct 4 and alkaline phosphatase. Conclusion: This is the first report on successful vitrification of isolated ICMs and the subsequent derivation of ESC colonies. Vitrification of isolated ICMs is a novel approach for preservation of the "stem cell source” material. The Author(s), 2010 Blastocyst nationallicence Cryopreservation nationallicence Human embryonic stem cells nationallicence Inner cell mass nationallicence Vitrification nationallicence Desai Nina Department of OB-GYN, Cleveland Clinic Foundation, Women's Health Institute, Beachwood, OH, USA aut Xu Jing Department of OB-GYN, Cleveland Clinic Foundation, Women's Health Institute, Beachwood, OH, USA aut Tsulaia Tamara Department of OB-GYN, Cleveland Clinic Foundation, Women's Health Institute, Beachwood, OH, USA aut Szeptycki-Lawson Julia Department of OB-GYN, Cleveland Clinic Foundation, Women's Health Institute, Beachwood, OH, USA aut AbdelHafez Faten Department of OB-GYN, Cleveland Clinic Foundation, Women's Health Institute, Beachwood, OH, USA aut Goldfarb James Department of OB-GYN, Cleveland Clinic Foundation, Women's Health Institute, Beachwood, OH, USA aut Falcone Tommaso Department of OB-GYN, Cleveland Clinic Foundation, Women's Health Institute, Beachwood, OH, USA aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/2(2011-02-01), 93-99 1058-0468 28:2<93 2011 28 10815 https://doi.org/10.1007/s10815-010-9500-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9500-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Desai Nina Department of OB-GYN, Cleveland Clinic Foundation, Women's Health Institute, Beachwood, OH, USA aut NATIONALLICENCE 700 1- Xu Jing Department of OB-GYN, Cleveland Clinic Foundation, Women's Health Institute, Beachwood, OH, USA aut NATIONALLICENCE 700 1- Tsulaia Tamara Department of OB-GYN, Cleveland Clinic Foundation, Women's Health Institute, Beachwood, OH, USA aut NATIONALLICENCE 700 1- Szeptycki-Lawson Julia Department of OB-GYN, Cleveland Clinic Foundation, Women's Health Institute, Beachwood, OH, USA aut NATIONALLICENCE 700 1- AbdelHafez Faten Department of OB-GYN, Cleveland Clinic Foundation, Women's Health Institute, Beachwood, OH, USA aut NATIONALLICENCE 700 1- Goldfarb James Department of OB-GYN, Cleveland Clinic Foundation, Women's Health Institute, Beachwood, OH, USA aut NATIONALLICENCE 700 1- Falcone Tommaso Department of OB-GYN, Cleveland Clinic Foundation, Women's Health Institute, Beachwood, OH, USA aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/2(2011-02-01), 93-99 1058-0468 28:2<93 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer