caa a22 4500 445817917 CHVBK 20180317145234.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10815-010-9495-3 doi (NATIONALLICENCE)springer-10.1007/s10815-010-9495-3 Assisted hatching in assisted reproduction: a state of the art [Elektronische Daten] [Mohamad Hammadeh, Constanze Fischer-Hammadeh, Khaled Ali] The World Health Organization estimates that one in six couples experience some delay in conception and an increasing number require treatment by the assisted conception (AC) procedures of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI).The implantation rate of embryos resulting from in vitro fertilization cycles is generally less than 20%. The exposure of oocytes and embryos to the artificial conditions of in vitro culture may have negative effects on the embryo's ability to undergo normal hatching, resulting in low rates of implantation following IVF and embryo transfer. Human embryos resulting from superovulation develop more slowly in vitro compared to embryos in vivo, manifest a relatively high degree of cytogenetic abnormalities and undergo cellular fragmentation. Artificially disrupting the zona pellucida is known as assisted hatching (AH) and there is some evidence that embryos that have undergone zona manipulation for assisted hatching tend to implant one day earlier than unhatched embryos. A variety of techniques have since been employed to assist embryo hatching, including partial mechanical zona dissection, zona drilling and zona thinning, making use of acid tyrodes, proteinases, piezon vibrator manipulators and lasers. This review will consider the impact of IVF conditions on zona pellucida physiology, zona hardening, different techniques of assisted hatching, who may benefit from assisted hatching and potential hazards. Springer Science+Business Media, LLC, 2010 Assisted hatching nationallicence Zona pellucida nationallicence Implantation nationallicence LASER nationallicence Zona drilling nationallicence Tyrode's solution nationallicence Hammadeh Mohamad Obstetrics and Gynaecology Department, Assisted Reproduction Technology Unit, University of Saarland, Homburg/Saar, Germany aut Fischer-Hammadeh Constanze Obstetrics and Gynaecology Department, Assisted Reproduction Technology Unit, University of Saarland, Homburg/Saar, Germany aut Ali Khaled Obstetrics and Gynaecology Department, Al-Azhar University, Assuit, Egypt aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/2(2011-02-01), 119-128 1058-0468 28:2<119 2011 28 10815 https://doi.org/10.1007/s10815-010-9495-3 text/html Onlinezugriff via DOI 1 editorial jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9495-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hammadeh Mohamad Obstetrics and Gynaecology Department, Assisted Reproduction Technology Unit, University of Saarland, Homburg/Saar, Germany aut NATIONALLICENCE 700 1- Fischer-Hammadeh Constanze Obstetrics and Gynaecology Department, Assisted Reproduction Technology Unit, University of Saarland, Homburg/Saar, Germany aut NATIONALLICENCE 700 1- Ali Khaled Obstetrics and Gynaecology Department, Al-Azhar University, Assuit, Egypt aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/2(2011-02-01), 119-128 1058-0468 28:2<119 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer