caa a22 4500 445817925 CHVBK 20180317145234.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10815-010-9496-2 doi (NATIONALLICENCE)springer-10.1007/s10815-010-9496-2 Evaluation of maturation competence of metaphase II oocytes in mice based on the distance between pericentriolar materials of meiotic spindle [Elektronische Daten] Distance of PCM during oocyte maturation [Chizuka Sakai, Yumi Hoshino, Yusuke Sato, Eimei Sato] Purpose: To ascertain whether metaphase II (MII) spindle shape influences oocyte competence, we examined the meiotic spindle organization in in vivo ovulated (IVO) oocytes and in spontaneously matured or follicle stimulating hormone (FSH)-induced oocytes. Methods: FSH-induced oocytes matured in Waymouth's MB752/1 or human tubal fluid (HTF) media and oocytes matured spontaneously in the basal medium were obtained, and spindles were detected by immunofluorescence. To evaluate the fertilization-associated differences in spindle morphology, we performed in vitro fertilization and analysed integrin mRNA expression. Results: The distance between the pericentriolar materials (PCMs) in oocytes matured under all conditions was initially more, but it reduced gradually and increased again thereafter. Therefore, oocytes exhibiting a reduction in the distance between PCMs had the highest development rate to blastocyst in each condition. Conclusion: These results indicate that the ‘maturation competence' of MII oocytes can be evaluated on the basis of the distance between PCMs. Springer Science+Business Media, LLC, 2010 In vitro maturation nationallicence Maturation competence nationallicence Meiotic spindle nationallicence Mouse oocyte nationallicence Pericentriolar materials nationallicence Sakai Chizuka Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 981-8555, Sendai, Japan aut Hoshino Yumi Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 981-8555, Sendai, Japan aut Sato Yusuke Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 981-8555, Sendai, Japan aut Sato Eimei Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 981-8555, Sendai, Japan aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/2(2011-02-01), 157-166 1058-0468 28:2<157 2011 28 10815 https://doi.org/10.1007/s10815-010-9496-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9496-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sakai Chizuka Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 981-8555, Sendai, Japan aut NATIONALLICENCE 700 1- Hoshino Yumi Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 981-8555, Sendai, Japan aut NATIONALLICENCE 700 1- Sato Yusuke Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 981-8555, Sendai, Japan aut NATIONALLICENCE 700 1- Sato Eimei Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 981-8555, Sendai, Japan aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/2(2011-02-01), 157-166 1058-0468 28:2<157 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer