caa a22 4500 445817933 CHVBK 20180317145234.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10815-010-9490-8 doi (NATIONALLICENCE)springer-10.1007/s10815-010-9490-8 Fertilization outcome, embryo development and birth after unstimulated IVM [Elektronische Daten] [Adriana Bos-Mikich, Marcelo Ferreira, Marcos Höher, Gerta Frantz, Norma Oliveira, Caroline Dutra, Nilo Frantz] Background/Purposes: A common observation in oocyte in vitro maturation (IVM) cycles is poor embryo quality. However, no study was dedicated to assess zygote and early cleavage embryo quality in IVM cycles. The objective of this study is to analyze fertilization outcome, embryo development and the resulting pregnancy and births in unstimulated IVM cycles. Methods: IVM oocytes were collected 36h post hCG and matured in vitro for 28-30h. All oocytes were inseminated by ICSI. Resulting zygotes and embryos were assessed on day-1, 2 and 3, when transfers were made. Results: The overall oocyte maturation and fertilization rates were 63% and 62%, respectively. Abnormal fertilization rate was 1.7%. Ninety five and 14.6% of the 2Pn zygotes reached the 2-cell and 8 -cell stage at day-2 and day-3, respectively. Embryo quality assessment on day-3 at transfer revealed that only 9% of the embryos were of very good quality. Most embryos showed developmental delay. An average of 3.29 embryos were transferred per patient resulting in implantation and clinical gestation rates of 16% and 32%, respectively. Overall 14 healthy babies were born and there is one ongoing pregnancy. Conclusion: Results show a significant rate of abnormal fertilization and poor embryo quality after IVM, which is reflected in a higher than average number of embryos being transferred. However, pregnancy, implantation and birth rates are reasonably high and allow us to consider IVM a valuable approach for the treatment of infertility in PCO or PCOS patients. Springer Science+Business Media, LLC, 2010 IVM nationallicence Embryo quality nationallicence Implantation nationallicence Pregnancy nationallicence Births nationallicence Bos-Mikich Adriana Department of Morphological Sciences, ICBS, Universidade Federal do Rio Grande do Sul, CEP: 90.050-170, Porto Alegre, RS, Brazil aut Ferreira Marcelo Nilo Frantz Research and Human Reproduction Centre, CEP: 90.480-003, Porto Alegre, RS, Brazil aut Höher Marcos Nilo Frantz Research and Human Reproduction Centre, CEP: 90.480-003, Porto Alegre, RS, Brazil aut Frantz Gerta Nilo Frantz Research and Human Reproduction Centre, CEP: 90.480-003, Porto Alegre, RS, Brazil aut Oliveira Norma Nilo Frantz Research and Human Reproduction Centre, CEP: 90.480-003, Porto Alegre, RS, Brazil aut Dutra Caroline Nilo Frantz Research and Human Reproduction Centre, CEP: 90.480-003, Porto Alegre, RS, Brazil aut Frantz Nilo Nilo Frantz Research and Human Reproduction Centre, CEP: 90.480-003, Porto Alegre, RS, Brazil aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/2(2011-02-01), 107-110 1058-0468 28:2<107 2011 28 10815 https://doi.org/10.1007/s10815-010-9490-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9490-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bos-Mikich Adriana Department of Morphological Sciences, ICBS, Universidade Federal do Rio Grande do Sul, CEP: 90.050-170, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Ferreira Marcelo Nilo Frantz Research and Human Reproduction Centre, CEP: 90.480-003, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Höher Marcos Nilo Frantz Research and Human Reproduction Centre, CEP: 90.480-003, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Frantz Gerta Nilo Frantz Research and Human Reproduction Centre, CEP: 90.480-003, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Oliveira Norma Nilo Frantz Research and Human Reproduction Centre, CEP: 90.480-003, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Dutra Caroline Nilo Frantz Research and Human Reproduction Centre, CEP: 90.480-003, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Frantz Nilo Nilo Frantz Research and Human Reproduction Centre, CEP: 90.480-003, Porto Alegre, RS, Brazil aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/2(2011-02-01), 107-110 1058-0468 28:2<107 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer