caa a22 4500 445817968 CHVBK 20180317145234.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10815-010-9493-5 doi (NATIONALLICENCE)springer-10.1007/s10815-010-9493-5 Spontaneous in vitro maturation and artificial activation of human germinal vesicle oocytes recovered from stimulated cycles [Elektronische Daten] [Laura Escrich, Noelia Grau, Amparo Mercader, Carmen Rubio, Antonio Pellicer, María-José Escribá] Purpose: To define germinal vesicles (GV) by morphometric and morphologic examination and by chromatin compaction and to assess their spontaneous nuclear and cytoplasmic competence. Materials: 131 GV were cultured for 42.7 ± 2.4h. Nuclear maturation was evaluated at four time points. Sixty-seven in vitro and twenty-five in vivo metaphase II (MII) were activated. Parthenotes with 2 PB and one pronucleus (NA) were studied for ploidy. Results: A total of 74.8% GV matured to MII: 55% at 21.4 ± 2.4h and 47.3% in the following 24h. Artificial activation induced NA in 79.2% of in vivo-MII and in 22.4% of in vitro-MII. All NA were haploid. Conclusions: GV spontaneously mature at the nuclear level. Their NA are haploid, but their cytoplasmic competence is compromised. Variables were not found to be predictors of oocyte competence, probably due to our population being homogeneous with respect to most of the variables studied. Springer Science+Business Media, LLC, 2010 Germinal vesicle nationallicence Humans nationallicence Oocyte activation nationallicence Spontaneous in vitro maturation nationallicence Escrich Laura Universitary Institute IVI Valencia, Valencia, Spain aut Grau Noelia Universitary Institute IVI Valencia, Valencia, Spain aut Mercader Amparo Universitary Institute IVI Valencia, Valencia, Spain aut Rubio Carmen Universitary Institute IVI Valencia, Valencia, Spain aut Pellicer Antonio Universitary Institute IVI Valencia, Valencia, Spain aut Escribá María-José Universitary Institute IVI Valencia, Valencia, Spain aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/2(2011-02-01), 111-117 1058-0468 28:2<111 2011 28 10815 https://doi.org/10.1007/s10815-010-9493-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9493-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Escrich Laura Universitary Institute IVI Valencia, Valencia, Spain aut NATIONALLICENCE 700 1- Grau Noelia Universitary Institute IVI Valencia, Valencia, Spain aut NATIONALLICENCE 700 1- Mercader Amparo Universitary Institute IVI Valencia, Valencia, Spain aut NATIONALLICENCE 700 1- Rubio Carmen Universitary Institute IVI Valencia, Valencia, Spain aut NATIONALLICENCE 700 1- Pellicer Antonio Universitary Institute IVI Valencia, Valencia, Spain aut NATIONALLICENCE 700 1- Escribá María-José Universitary Institute IVI Valencia, Valencia, Spain aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/2(2011-02-01), 111-117 1058-0468 28:2<111 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer