caa a22 4500 445817976 CHVBK 20180317145234.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10815-010-9491-7 doi (NATIONALLICENCE)springer-10.1007/s10815-010-9491-7 Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study [Elektronische Daten] [Mourad Assidi, Markus Montag, Katrin Van Der Ven, Marc-André Sirard] Purpose: To identify reliable genomic biomarkers expressed in cumulus cells that accurately and non-invasively predict the oocyte developmental competence and reinforce the already used morphological criteria. Methods: Eight consenting patients were selected for ovarian stimulation and ICSI procedures. Cumulus-oocyte complexes were transvaginally punctured and individually selected based on both good morphological criteria and high zona pellucida birefringence. Following ICSI, two 3-day embryos per patient were transferred. Pregnancy outcome was recorded and proven implantation was thereafter confirmed. Differential gene expression was assessed using two microarray platforms. Further real-time PCR validation, Ingenuity pathways analysis and intra-patient analysis were performed on 17 selected candidates. Results: Seven genes were differentially (p ≤ 0.05) associated to successful pregnancy and implantation. These biomarkers could be used to predict the oocyte developmental competence. Conclusions: These genomic markers are a powerful reinforcement of morphological approaches of oocyte selection. Their large-scale validation could increase pregnancy outcome and single embryo transfer efficiency. Springer Science+Business Media, LLC, 2010 Cumulus cells nationallicence Genomic biomarkers nationallicence Infertility nationallicence Oocyte developmental competence nationallicence Pregnancy nationallicence Zona pellucida birefringence nationallicence Assidi Mourad Centre de recherche en biologie de la reproduction, Département des Sciences Animales, Laval University, G1K 7P4, Ste-Foy, Québec, Canada aut Montag Markus Department of Gynecological Endocrinology and Reproductive Medicine, Bonn University, Bonn, Germany aut Van Der Ven Katrin Department of Gynecological Endocrinology and Reproductive Medicine, Bonn University, Bonn, Germany aut Sirard Marc-André Centre de recherche en biologie de la reproduction, Département des Sciences Animales, Laval University, G1K 7P4, Ste-Foy, Québec, Canada aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/2(2011-02-01), 173-188 1058-0468 28:2<173 2011 28 10815 https://doi.org/10.1007/s10815-010-9491-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-010-9491-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Assidi Mourad Centre de recherche en biologie de la reproduction, Département des Sciences Animales, Laval University, G1K 7P4, Ste-Foy, Québec, Canada aut NATIONALLICENCE 700 1- Montag Markus Department of Gynecological Endocrinology and Reproductive Medicine, Bonn University, Bonn, Germany aut NATIONALLICENCE 700 1- Van Der Ven Katrin Department of Gynecological Endocrinology and Reproductive Medicine, Bonn University, Bonn, Germany aut NATIONALLICENCE 700 1- Sirard Marc-André Centre de recherche en biologie de la reproduction, Département des Sciences Animales, Laval University, G1K 7P4, Ste-Foy, Québec, Canada aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/2(2011-02-01), 173-188 1058-0468 28:2<173 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer