caa a22 4500 445818093 CHVBK 20180317145235.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10815-011-9638-1 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9638-1 Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos [Elektronische Daten] [Shahram Jafari, Sayyed Hosseini, Mehdi Hajian, Mohsen Forouzanfar, Farnoosh Jafarpour, Parvaneh Abedi, Somayyeh Ostadhosseini, Hasan Abbasi, Hamid Gourabi, Abdolhossein Shahverdi, Ahmad Vosough, Maryam Anjomshoaa, Abd Haron, Norshariza Nordin, Halimatun Yaakub, Mohammad Nasr-Esfahani] Purpose: To investigate the effect of epigenetic modification on pattern, time and capacity of transcription activation of POU5F1, the key marker of pluripotency, in cloned bovine embryos. Methods: Bovine fibroblasts were stably transfected with POU5F1 promoter-driven enhanced green fluorescent protein (EGFP). This provided a visible marker to investigate the effect of post-activation treatment of cloned bovine embryos with trichostatin A (TSA) on time and capacity of POU5F1 expression and its subsequent effect on in vitro development of cloned bovine embryos. Results: Irrespective of TSA treatment, POU5F1 expression was not detected until 8-16 cell stage, but was detected in both inner cell mass and trophectoderm at the blastocyst stage. TSA treatment significantly increased POU5F1 expression, and the yield and quality of cloned embryo development compared to control. Conclusion: The POU5F1 expression of cloned embryos is strictly controlled by the stage of embryo development and may not be altered by TSA-mediated changes occur in DNA-methylation and histone-acetylation of the genome. Springer Science+Business Media, LLC, 2011 Epigenetic modification nationallicence POU5F1 nationallicence Bovine nationallicence Cloned embryos nationallicence Jafari Shahram Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia aut Hosseini Sayyed Department of Reproduction and Development, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran aut Hajian Mehdi Department of Reproduction and Development, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran aut Forouzanfar Mohsen Department of Basic Science, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran aut Jafarpour Farnoosh Department of Reproduction and Development, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran aut Abedi Parvaneh Department of Reproduction and Development, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran aut Ostadhosseini Somayyeh Department of Reproduction and Development, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran aut Abbasi Hasan Department of Reproduction and Development, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran aut Gourabi Hamid Department of Genetics, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran aut Shahverdi Abdolhossein Department of Reproduction and Development, Cell Sciences Research Center, ACECR, Isfahan, Iran aut Vosough Ahmad Department of Imaging, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran aut Anjomshoaa Maryam Department of Anatomical Science, University of Medical Science, Rafsanjan, Iran aut Haron Abd Department of Veterinary Clinical Studies, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia aut Nordin Norshariza Department of Obstetric and Gynaecology, Faculty of Health and Medicine, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia aut Yaakub Halimatun Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia aut Nasr-Esfahani Mohammad Department of Reproduction and Development, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/11(2011-11-01), 1119-1127 1058-0468 28:11<1119 2011 28 10815 https://doi.org/10.1007/s10815-011-9638-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9638-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jafari Shahram Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia aut NATIONALLICENCE 700 1- Hosseini Sayyed Department of Reproduction and Development, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran aut NATIONALLICENCE 700 1- Hajian Mehdi Department of Reproduction and Development, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran aut NATIONALLICENCE 700 1- Forouzanfar Mohsen Department of Basic Science, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran aut NATIONALLICENCE 700 1- Jafarpour Farnoosh Department of Reproduction and Development, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran aut NATIONALLICENCE 700 1- Abedi Parvaneh Department of Reproduction and Development, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran aut NATIONALLICENCE 700 1- Ostadhosseini Somayyeh Department of Reproduction and Development, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran aut NATIONALLICENCE 700 1- Abbasi Hasan Department of Reproduction and Development, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran aut NATIONALLICENCE 700 1- Gourabi Hamid Department of Genetics, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran aut NATIONALLICENCE 700 1- Shahverdi Abdolhossein Department of Reproduction and Development, Cell Sciences Research Center, ACECR, Isfahan, Iran aut NATIONALLICENCE 700 1- Vosough Ahmad Department of Imaging, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran aut NATIONALLICENCE 700 1- Anjomshoaa Maryam Department of Anatomical Science, University of Medical Science, Rafsanjan, Iran aut NATIONALLICENCE 700 1- Haron Abd Department of Veterinary Clinical Studies, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia aut NATIONALLICENCE 700 1- Nordin Norshariza Department of Obstetric and Gynaecology, Faculty of Health and Medicine, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia aut NATIONALLICENCE 700 1- Yaakub Halimatun Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia aut NATIONALLICENCE 700 1- Nasr-Esfahani Mohammad Department of Reproduction and Development, Cell Sciences Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, Iran aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/11(2011-11-01), 1119-1127 1058-0468 28:11<1119 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer