caa a22 4500 445818115 CHVBK 20180317145235.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10815-011-9634-5 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9634-5 Relationship between meiotic spindle characteristics in human oocytes and the timing of the first zygotic cleavage after intracytoplasmic sperm injection [Elektronische Daten] [Hiroyuki Tomari, Kou Honjou, Yumi Nagata, Toshitaka Horiuchi] Purpose: To investigate the relationship between meiotic spindle characteristics in human oocytes and the timing of the first zygotic cleavage after intracytoplasmic sperm injection (ICSI). Methods: Zygotes that had cleaved to two-cell stage by 27h post-ICSI were classified as early cleaving and the remainder as late cleaving. Meiotic spindle parameters previously imaged using the PolScope were compared between the two groups. Results: Of 384 embryos, 163 were classed as early cleaving and 221 as late cleaving. The rate of blastocyst formation or pregnancy by Day 2 embryo transfer was significantly higher following early cleavage than after late cleavage (52.4% vs. 24.4% or 32.6% vs. 11.4%). Spindle areas (108.0 vs. 89.8μm2), lengths (14.7 vs. 13.4μm) and PolScope retardance were also significantly greater in the early cleaving group. Conclusions: Meiotic spindle parameters determine the timing of the first zygotic cleavage and are strong indicators of human embryo developmental potential. Springer Science+Business Media, LLC, 2011 Early cleavage nationallicence Meiotic spindle nationallicence Embryo quality nationallicence PolScope nationallicence Human oocytes nationallicence Tomari Hiroyuki IVF Nagata Clinic, 810-0001, Fukuoka, Japan aut Honjou Kou IVF Nagata Clinic, 810-0001, Fukuoka, Japan aut Nagata Yumi IVF Nagata Clinic, 810-0001, Fukuoka, Japan aut Horiuchi Toshitaka Graduate School of Comprehensive Scientific Research, Prefectural University of Hiroshima, 727-0023, Hiroshima, Japan aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/11(2011-11-01), 1099-1104 1058-0468 28:11<1099 2011 28 10815 https://doi.org/10.1007/s10815-011-9634-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9634-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tomari Hiroyuki IVF Nagata Clinic, 810-0001, Fukuoka, Japan aut NATIONALLICENCE 700 1- Honjou Kou IVF Nagata Clinic, 810-0001, Fukuoka, Japan aut NATIONALLICENCE 700 1- Nagata Yumi IVF Nagata Clinic, 810-0001, Fukuoka, Japan aut NATIONALLICENCE 700 1- Horiuchi Toshitaka Graduate School of Comprehensive Scientific Research, Prefectural University of Hiroshima, 727-0023, Hiroshima, Japan aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/11(2011-11-01), 1099-1104 1058-0468 28:11<1099 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer