caa a22 4500 445818166 CHVBK 20180317145235.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10815-011-9631-8 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9631-8 Sperm DNA integrity assays: diagnostic and prognostic challenges and implications in management of infertility [Elektronische Daten] [Monis Shamsi, Syed Imam, Rima Dada] Sperm is not a simple carrier of paternal genetic information but its role extends clearly beyond fertilization. Integrity of sperm genome is an essential pre-requisite for birth of healthy offspring and evaluation of sperm should entail DNA integrity analysis. DNA integrity analysis is a better diagnostic and prognostic marker of sperm reproductive potential. Conventional semen analysis emphasizes on sperm concentration, viability, motility and morphology and has been proven to be a poor indicator of reproductive potential and pregnancy outcome. To overcome the drawbacks associated with conventional semen analysis more useful fertility tests and molecular biomarkers have been explored. Among the different tests which have evolved for assessing the sperm reproductive potential, tests for sperm DNA quality are most promising. Sperm DNA damage has been closely associated with numerous indicators of reproductive health including fertilization, embryo quality, implantation, spontaneous abortion, congenital malformations and childhood diseases. It therefore has great potential as a prognostic test for both in vitro and in vivo conception. This review presents an updated account of tests that have better diagnostic and prognostic implications in the evaluation of sperm DNA damage. The basic principles, outline of methodology, advantage, disadvantage, clinical significance of each technique and implications of these tests have been discussed. The logistics of each test with respect to available resources and equipment in an andrology laboratory, the feasibility of performing these tests in routine diagnostic workup of infertile men and the opportunities and challenges provided by DNA testing in male fertility determination are also presented. Springer Science+Business Media, LLC, 2011 Sperm DNA integrity nationallicence Semen analysis nationallicence DNA integrity tests nationallicence Comet, TUNEL, SCSA nationallicence Acridine orange test nationallicence Comet assay nationallicence Toluidine blue staining nationallicence Chromomycin A-3 nationallicence Sperm chromatin dispersion nationallicence Infertility, recurrent spontaneous abortion, assisted reproduction technique nationallicence Shamsi Monis Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Science, New Delhi, India aut Imam Syed Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Science, New Delhi, India aut Dada Rima Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Science, New Delhi, India aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/11(2011-11-01), 1073-1085 1058-0468 28:11<1073 2011 28 10815 https://doi.org/10.1007/s10815-011-9631-8 text/html Onlinezugriff via DOI 1 review-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9631-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shamsi Monis Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Science, New Delhi, India aut NATIONALLICENCE 700 1- Imam Syed Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Science, New Delhi, India aut NATIONALLICENCE 700 1- Dada Rima Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Science, New Delhi, India aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/11(2011-11-01), 1073-1085 1058-0468 28:11<1073 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer