caa a22 4500 445818190 CHVBK 20180317145235.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s10815-011-9632-7 doi (NATIONALLICENCE)springer-10.1007/s10815-011-9632-7 The prevalence of common CFTR mutations in Iranian infertile men with non-CAVD obstructive azoospermia by using ARMS PCR techniques [Elektronische Daten] [Kyumars Safinejad, Mojtaba Darbouy, Sayed Kalantar, Sirus Zeinali, Reza Mirfakhraie, Leila Yadegar, Masoud Houshmand] Purpose: To evaluate five common cystic fibrosis trans-membrane conductance regulator (CFTR) mutations (ΔF508, G542X, R117H, W1282X and N1303K) in the Iranian infertile men with noncongenital absence of vas deferens (CAVD) obstructive azoospermia. Methods: The common CFTR gene mutations were tested on blood samples from 53 infertile men with non-CAVD obstructive azoospermia and 50 normal men as control individuals. Genomic DNA is extracted from the whole blood and the common CFTR mutations have been detected by the amplification refractory mutation system (ARMS) techniques. Results: The common CFTR mutations were found positive in 5/53)9.43%(for ΔF508 and 4/53)7.55%(for G542X mutation of all patients tested. Also, no CFTR mutations were detected in the normal men. Conclusion: The common CFTR mutations were detected in 9/53(17%) infertile men with non-CAVD obstructive azoospermia. Pre-treatment CFTR mutation analysis remains critical to distinguish cystic fibrosis (CF) genotypes for men with non CAVD obstructive azoospermia. Springer Science+Business Media, LLC, 2011 Obstructive azoospermia nationallicence Common CFTR gene mutations nationallicence Non CAVD nationallicence Infertile men nationallicence ARMS PCR nationallicence Safinejad Kyumars Fars Science and Research branch, Islamic Azad University, Shiraz, Iran aut Darbouy Mojtaba Fars Science and Research branch, Islamic Azad University, Shiraz, Iran aut Kalantar Sayed Research & Clinical Centre for Infertility, Shahid Sadoughi Medical Sciences University Yazd, Yazd, Iran aut Zeinali Sirus Department of Molecular Medicine, Pastor Institute of Iran, Tehran, Iran aut Mirfakhraie Reza Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences and Health Services, Tehran, Iran aut Yadegar Leila Department of Biology, Faculty of Science, Payam Noor University (PNU), Tehran, Iran aut Houshmand Masoud National Institute of Genetic Engineering and Biotechnology, Tehran, Iran aut Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/11(2011-11-01), 1087-1090 1058-0468 28:11<1087 2011 28 10815 https://doi.org/10.1007/s10815-011-9632-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10815-011-9632-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Safinejad Kyumars Fars Science and Research branch, Islamic Azad University, Shiraz, Iran aut NATIONALLICENCE 700 1- Darbouy Mojtaba Fars Science and Research branch, Islamic Azad University, Shiraz, Iran aut NATIONALLICENCE 700 1- Kalantar Sayed Research & Clinical Centre for Infertility, Shahid Sadoughi Medical Sciences University Yazd, Yazd, Iran aut NATIONALLICENCE 700 1- Zeinali Sirus Department of Molecular Medicine, Pastor Institute of Iran, Tehran, Iran aut NATIONALLICENCE 700 1- Mirfakhraie Reza Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences and Health Services, Tehran, Iran aut NATIONALLICENCE 700 1- Yadegar Leila Department of Biology, Faculty of Science, Payam Noor University (PNU), Tehran, Iran aut NATIONALLICENCE 700 1- Houshmand Masoud National Institute of Genetic Engineering and Biotechnology, Tehran, Iran aut NATIONALLICENCE 773 0- Journal of Assisted Reproduction and Genetics Springer US; http://www.springer-ny.com 28/11(2011-11-01), 1087-1090 1058-0468 28:11<1087 2011 28 10815 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer