caa a22 4500 445818271 CHVBK 20180317145235.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00792-010-0339-y doi (NATIONALLICENCE)springer-10.1007/s00792-010-0339-y Temperature and pH controls on glycerol dibiphytanyl glycerol tetraether lipid composition in the hyperthermophilic crenarchaeon Acidilobus sulfurireducens [Elektronische Daten] [Eric Boyd, Ann Pearson, Yundan Pi, Wen-Jun Li, Yi Zhang, Liu He, Chuanlun Zhang, Gill Geesey] Cyclization in glycerol dibiphytanyl glycerol tetraethers (GDGTs) results in internal cyclopentane moieties which are believed to confer thermal stability to crenarchaeal membranes. While the average number of rings per GDGT lipid (ring index) is positively correlated with temperature in many temperate environments, poor correlations are often observed in geothermal environments, suggesting that additional parameters may influence GDGT core lipid composition in these systems. However, the physical and chemical parameters likely to influence GDGT cyclization which are often difficult to decouple in geothermal systems, making it challenging to assess their influence on lipid composition. In the present study, the influence of temperature (range 65-81°C), pH (range 3.0-5.0), and ionic strength (range 10.1-55.7mM) on GDGT core lipid composition was examined in the hyperthermoacidophile Acidilobus sulfurireducens, a crenarchaeon originally isolated from a geothermal spring in Yellowstone National Park, Wyoming. When cultivated under defined laboratory conditions, the composition of individual and total GDGTs varied significantly with temperature and to a lesser extent with the pH of the growth medium. Ionic strength over the range of values tested did not influence GDGT composition. The GDGT core lipid ring index was positively correlated with temperature and negatively correlated with pH, suggesting that A. sulfurireducens responds to increasing temperature and acidity by increasing the number of cyclopentyl rings in GDGT core membrane lipids. Springer, 2010 Glycerol dibiphytanyl glycerol tetraether nationallicence GDGT nationallicence Yellowstone nationallicence pH nationallicence Temperature nationallicence Crenarchaea nationallicence Boyd Eric Department of Microbiology, Montana State University, 109 Lewis Hall, 59717, Bozeman, MT, USA aut Pearson Ann Department of Earth and Planetary Sciences, Harvard University, 02138, Cambridge, MA, USA aut Pi Yundan Department of Earth and Planetary Sciences, Harvard University, 02138, Cambridge, MA, USA aut Li Wen-Jun Yunnan Institute of Microbiology, Yunnan University, 650091, Kunming, China aut Zhang Yi Department of Geology and Geophysics, Yale University, 06520, New Haven, CT, USA aut He Liu State Key Laboratory of Marine Geology, Tongji University, 200092, Shanghai, China aut Zhang Chuanlun State Key Laboratory of Marine Geology, Tongji University, 200092, Shanghai, China aut Geesey Gill Department of Microbiology, Montana State University, 109 Lewis Hall, 59717, Bozeman, MT, USA aut Extremophiles Springer Japan 15/1(2011-01-01), 59-65 1431-0651 15:1<59 2011 15 792 https://doi.org/10.1007/s00792-010-0339-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-010-0339-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boyd Eric Department of Microbiology, Montana State University, 109 Lewis Hall, 59717, Bozeman, MT, USA aut NATIONALLICENCE 700 1- Pearson Ann Department of Earth and Planetary Sciences, Harvard University, 02138, Cambridge, MA, USA aut NATIONALLICENCE 700 1- Pi Yundan Department of Earth and Planetary Sciences, Harvard University, 02138, Cambridge, MA, USA aut NATIONALLICENCE 700 1- Li Wen-Jun Yunnan Institute of Microbiology, Yunnan University, 650091, Kunming, China aut NATIONALLICENCE 700 1- Zhang Yi Department of Geology and Geophysics, Yale University, 06520, New Haven, CT, USA aut NATIONALLICENCE 700 1- He Liu State Key Laboratory of Marine Geology, Tongji University, 200092, Shanghai, China aut NATIONALLICENCE 700 1- Zhang Chuanlun State Key Laboratory of Marine Geology, Tongji University, 200092, Shanghai, China aut NATIONALLICENCE 700 1- Geesey Gill Department of Microbiology, Montana State University, 109 Lewis Hall, 59717, Bozeman, MT, USA aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/1(2011-01-01), 59-65 1431-0651 15:1<59 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer