caa a22 4500 44581828X CHVBK 20180317145235.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00792-010-0338-z doi (NATIONALLICENCE)springer-10.1007/s00792-010-0338-z Single-stranded DNA binding activity of XPBI, but not XPBII, from Sulfolobus tokodaii causes double-stranded DNA melting [Elektronische Daten] [Xiaoqing Ma, Ye Hong, Wenyuan Han, Duohong Sheng, Jinfeng Ni, Guihua Hou, Yulong Shen] XPB helicase is the largest subunit of transcription factor IIH (TFIIH), a ten-subunit protein complex essential for transcription initiation and nucleotide excision repair (NER) in Eukarya. Two XPB homologues (XPBI and XPBII) are present in the genome of most crenarchaeota, one of the two major phyla of archaea; however, the biochemical properties have not been fully characterized and their cellular roles have not been clearly defined. Here, we report that XPBI from the hyperthermophilic crenarchaeon Sulfolobus tokodaii (StoXPBI) is able to destabilize double-stranded DNA (dsDNA) helix independent of ATP (designated as dsDNA melting activity). This activity is inhibited by single-stranded DNA (ssDNA) and relies on the unique N-terminal domain of StoXPBI, which is also likely responsible for the intrinsic strong ssDNA binding activity of StoXPBI as revealed by deletion analysis. We demonstrate that the ATPase activity of StoXPBII is remarkably stimulated by StoBax1, a nuclease partner of StoXPBII. The role of the unique dsDNA melting activity of XPBI in NER in archaea was discussed. Springer, 2010 Archaea nationallicence Nucleotide excision repair nationallicence Sulfolobus tokodaii nationallicence XPB nationallicence Bax1 nationallicence Ma Xiaoqing State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Rd., 250100, Jinan, People's Republic of China aut Hong Ye State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Rd., 250100, Jinan, People's Republic of China aut Han Wenyuan State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Rd., 250100, Jinan, People's Republic of China aut Sheng Duohong State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Rd., 250100, Jinan, People's Republic of China aut Ni Jinfeng State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Rd., 250100, Jinan, People's Republic of China aut Hou Guihua School of Medicine, Shandong University, 250012, Jinan, People's Republic of China aut Shen Yulong State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Rd., 250100, Jinan, People's Republic of China aut Extremophiles Springer Japan 15/1(2011-01-01), 67-76 1431-0651 15:1<67 2011 15 792 https://doi.org/10.1007/s00792-010-0338-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-010-0338-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ma Xiaoqing State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Rd., 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Hong Ye State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Rd., 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Han Wenyuan State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Rd., 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Sheng Duohong State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Rd., 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Ni Jinfeng State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Rd., 250100, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Hou Guihua School of Medicine, Shandong University, 250012, Jinan, People's Republic of China aut NATIONALLICENCE 700 1- Shen Yulong State Key Laboratory of Microbial Technology, Shandong University, 27 Shanda Nan Rd., 250100, Jinan, People's Republic of China aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/1(2011-01-01), 67-76 1431-0651 15:1<67 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer