caa a22 4500 445818298 CHVBK 20180317145235.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00792-010-0333-4 doi (NATIONALLICENCE)springer-10.1007/s00792-010-0333-4 Bacterial diversity of soil in the vicinity of Pindari glacier, Himalayan mountain ranges, India, using culturable bacteria and soil 16S rRNA gene clones [Elektronische Daten] [S. Shivaji, M. Pratibha, B. Sailaja, K. Hara Kishore, Ashish Singh, Z. Begum, Uttam Anarasi, S. Prabagaran, G. Reddy, T. Srinivas] Three 16S rRNA gene clone libraries (P1L, P4L and P8L) were constructed using three soil samples (P1S, P4S and P8S) collected near Pindari glacier, Himalayas. The three libraries yielded a total of 703 clones. Actinobacteria, Firmicutes and Proteobacteria were common to the three libraries. In addition to the above P1L and P8L shared the phyla Acidobacteria, Bacteroidetes, Gemmatimonadetes and Planctomycetes. Phyla Chlamydiae, Chlorobi, Chloroflexi, Dictyoglomi, Fibrobacteres, Nitrospirae, Verrucomicrobia, candidate division SPAM and candidate TM7s TM7a phylum were present only in P1L. Rarefaction analysis indicated that the bacterial diversity in P4S and P8S soil samples was representative of the sample. Principal component analysis (PCA) revealed that P1S and P8S were different from P4S soil sample. PCA also indicated that arsenic content, pH, Cr and altitude influence the observed differences in the percentage of specific OTUs in the three 16S rRNA gene clone libraries. The observed bacterial diversity was similar to that observed for other Himalayan and non-polar cold habitats. A total of 40 strains of bacteria were isolated from the above three soil samples and based on the morphology 20 bacterial strains were selected for further characterization. The 20 bacteria belonged to 12 different genera. All the isolates were psychro-, halo- and alkalitolerant. Amylase and urease activities were detected in majority of the strains but lipase and protease activities were not detected. Long chain, saturated, unsaturated and branched fatty acids were predominant in the psychrotolerant bacteria. Springer, 2010 Himalayan glacier nationallicence Pindari glacier nationallicence 16S rRNA gene clone library nationallicence Psychrophiles nationallicence Psychrophilic enzymes nationallicence Cellular fatty acids nationallicence Shivaji S. Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut Pratibha M. Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut Sailaja B. Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut Hara Kishore K. Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut Singh Ashish Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut Begum Z. Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut Anarasi Uttam Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut Prabagaran S. Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut Reddy G. Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut Srinivas T. Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut Extremophiles Springer Japan 15/1(2011-01-01), 1-22 1431-0651 15:1<1 2011 15 792 https://doi.org/10.1007/s00792-010-0333-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-010-0333-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shivaji S. Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut NATIONALLICENCE 700 1- Pratibha M. Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut NATIONALLICENCE 700 1- Sailaja B. Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut NATIONALLICENCE 700 1- Hara Kishore K. Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut NATIONALLICENCE 700 1- Singh Ashish Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut NATIONALLICENCE 700 1- Begum Z. Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut NATIONALLICENCE 700 1- Anarasi Uttam Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut NATIONALLICENCE 700 1- Prabagaran S. Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut NATIONALLICENCE 700 1- Reddy G. Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut NATIONALLICENCE 700 1- Srinivas T. Centre for Cellular and Molecular Biology, Uppal Road, 500007, Hyderabad, India aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/1(2011-01-01), 1-22 1431-0651 15:1<1 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer