caa a22 4500 445818336 CHVBK 20180317145235.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00792-011-0398-8 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0398-8 Comparison of bacterial diversity in proglacial soil from Kafni Glacier, Himalayan Mountain ranges, India, with the bacterial diversity of other glaciers in the world [Elektronische Daten] [T. Srinivas, S. Singh, Suman Pradhan, M. Pratibha, K. Kishore, Ashish Singh, Z. Begum, S. Prabagaran, G. Reddy, S. Shivaji] Two 16S rRNA gene clone libraries (KF and KS) were constructed using two soil samples (K7s and K8s) collected near Kafni Glacier, Himalayas. The two libraries yielded a total of 648 clones. Phyla Actinobacteria, Bacteroidetes, Chloroflexi Firmicutes, Proteobacteria, Spirochaetae, Tenericutes and Verrucomicrobia were common to the two libraries. Phyla Acidobacteria, Chlamydiae and Nitrospirae were present only in KF library, whereas Lentisphaerae and TM7 were detected only in KS. In the two libraries, clones belonging to phyla Bacteroidetes and Proteobacteria were the most predominant. Principal component analysis (PCA) revealed that KF and KS were different and arsenic content influenced the differences in the percentage of OTUs. PCA indicated that high water content in the K8s sample results in high total bacterial count. PCA also indicated that bacterial diversity of KF and KS was similar to soils from the Pindari Glacier, Himalayas; Samoylov Island, Siberia; Schrimacher Oasis, Antarctica and Siberian tundra. The eleven bacterial strains isolated from the above two soil samples were phylogenetically related to six different genera. All the isolates were psychro-, halo- and alkalitolerant. Amylase, lipase and urease activities were detected in the majority of the strains. Long chain, saturated, unsaturated and branched fatty acids were predominant in the psychrotolerant bacteria. Springer, 2011 Himalayan Mountain ranges nationallicence Kafni Glacier nationallicence 16S rRNA gene clone library nationallicence Psychrophilic enzymes nationallicence Cellular fatty acids nationallicence Srinivas T. Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut Singh S. National Centre for Antarctic Ocean Research, Headland Sada, 403 804, Vasco-da-Gama, Goa, India aut Pradhan Suman Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut Pratibha M. Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut Kishore K. Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut Singh Ashish Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut Begum Z. Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut Prabagaran S. Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut Reddy G. Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut Shivaji S. Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut Extremophiles Springer Japan 15/6(2011-11-01), 673-690 1431-0651 15:6<673 2011 15 792 https://doi.org/10.1007/s00792-011-0398-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0398-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Srinivas T. Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut NATIONALLICENCE 700 1- Singh S. National Centre for Antarctic Ocean Research, Headland Sada, 403 804, Vasco-da-Gama, Goa, India aut NATIONALLICENCE 700 1- Pradhan Suman Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut NATIONALLICENCE 700 1- Pratibha M. Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut NATIONALLICENCE 700 1- Kishore K. Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut NATIONALLICENCE 700 1- Singh Ashish Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut NATIONALLICENCE 700 1- Begum Z. Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut NATIONALLICENCE 700 1- Prabagaran S. Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut NATIONALLICENCE 700 1- Reddy G. Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut NATIONALLICENCE 700 1- Shivaji S. Centre for Cellular and Molecular Biology, Uppal Road, 500 007, Hyderabad, India aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/6(2011-11-01), 673-690 1431-0651 15:6<673 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer