caa a22 4500 445818360 CHVBK 20180317145236.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00792-011-0399-7 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0399-7 Natronoflexus pectinivorans gen. nov. sp. nov., an obligately anaerobic and alkaliphilic fermentative member of Bacteroidetes from soda lakes [Elektronische Daten] [D. Sorokin, A. Panteleeva, T. Tourova, E. Kaparullina, G. Muyzer] Anaerobic enrichment with pectin at pH 10 and moderate salinity inoculated with sediments from soda lakes of the Kulunda Steppe (Altai, Russia) resulted in the isolation of a novel member of the Bacteroidetes, strain AP1T. The cells are long, flexible, Gram-negative rods forming pink carotenoids. The isolate is an obligate anaerobe, fermenting various carbohydrates to acetate and succinate. It can hydrolyze and utilize pectin, xylan, starch, laminarin and pullulan as growth substrates. Growth is possible in a pH range from 8 to 10.5, with an optimum at pH 9.5, and at a salinity range from 0.1 to 2M Na+. Phylogenetic analysis based on 16S rRNA sequences placed the isolate into the phylum Bacteroidetes as a separate lineage within the family Marinilabilaceae. On the basis of distinct phenotype and phylogeny, the soda lake isolate AP1T is proposed to be assigned in a new genus and species Natronoflexus pectinivorans (=DSM24179T=UNIQEM U807T). The Author(s), 2011 Natronoflexus pectinivorans nationallicence Pectin nationallicence Haloalkaliphilic nationallicence Soda lakes nationallicence Sorokin D. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117811, Moscow, Russia aut Panteleeva A. Bioengineering Centre, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/1, 117811, Moscow, Russia aut Tourova T. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117811, Moscow, Russia aut Kaparullina E. Institute of Physiology and Biochemistry of Microorganisms, Russian Academy of Sciences, Puschino, Russia aut Muyzer G. Department of Biotechnology, Environmental Biotechnology Group, Delft University of Technology, Delft, The Netherlands aut Extremophiles Springer Japan 15/6(2011-11-01), 691-696 1431-0651 15:6<691 2011 15 792 https://doi.org/10.1007/s00792-011-0399-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0399-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sorokin D. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117811, Moscow, Russia aut NATIONALLICENCE 700 1- Panteleeva A. Bioengineering Centre, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/1, 117811, Moscow, Russia aut NATIONALLICENCE 700 1- Tourova T. Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117811, Moscow, Russia aut NATIONALLICENCE 700 1- Kaparullina E. Institute of Physiology and Biochemistry of Microorganisms, Russian Academy of Sciences, Puschino, Russia aut NATIONALLICENCE 700 1- Muyzer G. Department of Biotechnology, Environmental Biotechnology Group, Delft University of Technology, Delft, The Netherlands aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/6(2011-11-01), 691-696 1431-0651 15:6<691 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer