caa a22 4500 445818379 CHVBK 20180317145236.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00792-011-0396-x doi (NATIONALLICENCE)springer-10.1007/s00792-011-0396-x Diversity and phylogeny of the ectoine biosynthesis genes in aerobic, moderately halophilic methylotrophic bacteria [Elektronische Daten] [Alexander Reshetnikov, Valentina Khmelenina, Ildar Mustakhimov, Marina Kalyuzhnaya, Mary Lidstrom, Yuri Trotsenko] The genes of ectoine biosynthesis pathway were identified in six species of aerobic, slightly halophilic bacteria utilizing methane, methanol or methylamine. Two types of ectoine gene cluster organization were revealed in the methylotrophs. The gene cluster ectABC coding for diaminobutyric acid (DABA) acetyltransferase (EctA), DABA aminotransferase (EctB) and ectoine synthase (EctC) was found in methanotrophs Methylobacter marinus 7C and Methylomicrobium kenyense AMO1T. In methanotroph Methylomicrobium alcaliphilum ML1, methanol-utilizers Methylophaga thalassica 33146T , Methylophaga alcalica M8 and methylamine-utilizer Methylarcula marina h1T, the genes forming the ectABC-ask operon are preceded by ectR, encoding a putative transcriptional regulatory protein EctR. Phylogenetic relationships of the Ect proteins do not correlate with phylogenetic affiliation of the strains, thus implying that the ability of methylotrophs to produce ectoine is most likely the result of a horizontal transfer event. Springer, 2011 Methylotrophic bacteria nationallicence Methanotrophs nationallicence Compatible solutes nationallicence Ectoine biosynthesis genes nationallicence Reshetnikov Alexander Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Moscow region, Russia aut Khmelenina Valentina Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Moscow region, Russia aut Mustakhimov Ildar Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Moscow region, Russia aut Kalyuzhnaya Marina Department of Microbiology, University of Washington, Box 355014, Seattle, WA, USA aut Lidstrom Mary Department of Chemical Engineering, University of Washington, Box 355014, Seattle, WA, USA aut Trotsenko Yuri Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Moscow region, Russia aut Extremophiles Springer Japan 15/6(2011-11-01), 653-663 1431-0651 15:6<653 2011 15 792 https://doi.org/10.1007/s00792-011-0396-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0396-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reshetnikov Alexander Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Moscow region, Russia aut NATIONALLICENCE 700 1- Khmelenina Valentina Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Moscow region, Russia aut NATIONALLICENCE 700 1- Mustakhimov Ildar Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Moscow region, Russia aut NATIONALLICENCE 700 1- Kalyuzhnaya Marina Department of Microbiology, University of Washington, Box 355014, Seattle, WA, USA aut NATIONALLICENCE 700 1- Lidstrom Mary Department of Chemical Engineering, University of Washington, Box 355014, Seattle, WA, USA aut NATIONALLICENCE 700 1- Trotsenko Yuri Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290, Pushchino, Moscow region, Russia aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/6(2011-11-01), 653-663 1431-0651 15:6<653 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer