caa a22 4500 445818417 CHVBK 20180317145236.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00792-011-0400-5 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0400-5 Rhamnolipid-producing thermophilic bacteria of species Thermus and Meiothermus [Elektronische Daten] [Tomáš Řezanka, Lucie Siristova, Karel Sigler] Novel rhamnolipid-producing strains of three thermophilic bacteria, Thermus sp., T. aquaticus and Meiothermus ruber were identified that have not been previously described as rhamnolipid producers. Rhamnolipids were extracted from supernatant and further purified by thin-layer chromatography. Mass spectrometry with negative electrospray ionization revealed 77 rhamnolipid homologues varying in chain length and unsaturation. Tandem mass spectrometry identified mono-rhamnolipid and di-rhamnolipid homologues containing one or two 3-hydroxy-fatty acids, saturated, monounsaturated or diunsaturated, even- or odd-chain, up to unusual long chains with 24 carbon atoms. The stereochemistry of rhamnose was L and that of 3-hydroxy-fatty acids was R, the position of double bonds in monoenoic acids was cis ω-9. All three strains produced a rhamnolipid that differs in structure from Pseudomonas aeruginosa rhamnolipids and exhibits excellent surfactant properties. Importantly, in comparison to P. aeruginosa both strains, i.e., Thermus and Meiothermus, are Biosafety level 1 microorganisms and are not pathogenic to humans. Springer, 2011 Thermus sp nationallicence T. aquaticus nationallicence Meiothermus ruber nationallicence Rhamnolipids nationallicence Tandem mass spectrometry nationallicence Biosurfactant nationallicence Řezanka Tomáš Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague 4, Czech Republic aut Siristova Lucie Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology Prague, 166 28, Prague, Czech Republic aut Sigler Karel Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague 4, Czech Republic aut Extremophiles Springer Japan 15/6(2011-11-01), 697-709 1431-0651 15:6<697 2011 15 792 https://doi.org/10.1007/s00792-011-0400-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0400-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Řezanka Tomáš Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague 4, Czech Republic aut NATIONALLICENCE 700 1- Siristova Lucie Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology Prague, 166 28, Prague, Czech Republic aut NATIONALLICENCE 700 1- Sigler Karel Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20, Prague 4, Czech Republic aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/6(2011-11-01), 697-709 1431-0651 15:6<697 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer