caa a22 4500 445818433 CHVBK 20180317145236.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00792-011-0368-1 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0368-1 Uncultured archaea dominate in the thermal groundwater of Uzon Caldera, Kamchatka [Elektronische Daten] [Andrey Mardanov, Vadim Gumerov, Alexey Beletsky, Anna Perevalova, Gennady Karpov, Elizaveta Bonch-Osmolovskaya, Nikolai Ravin] The thermoacidophilic microbial community inhabiting the groundwater with pH 4.0 and temperature 50°C at the East Thermal Field of Uzon Caldera, Kamchatka, was examined using pyrosequencing of the V3 region of the 16S rRNA gene. Bacteria comprise about 30% of microorganisms and are represented primarily by aerobic lithoautotrophs using the energy sources of volcanic origin—thermoacidophilic methanotrophs of the phylum Verrucomicrobia and Acidithiobacillus spp. oxidising metals and reduced sulfur compounds. More than 70% of microbial population in this habitat were represented by archaea, in majority affiliated with "uncultured” lineages. The most numerous group (39% of all archaea) represented a novel division in the phylum Euryarchaeota related to the order Thermoplasmatales. Another abundant group (33% of all archaea) was related to MCG1 lineage of the phylum Crenarchaeota, originally detected in the Yellowstone hot spring as the environmental clone pJP89. The organisms belonging to these two groups are widely spread in hydrothermal environments worldwide. These data indicate an important environmental role of these two archaeal groups and should stimulate the investigation of their metabolism by cultivation or metagenomic approaches. Springer, 2011 Microbial community nationallicence Thermoacidophiles nationallicence Pyrosequencing nationallicence Thermal groundwater nationallicence Uncultured archaea nationallicence Uzon Caldera nationallicence Mardanov Andrey Centre "Bioengineering”, Russian Academy of Sciences, Prosp. 60-let Oktyabrya, bld.7-1, 117312, Moscow, Russia aut Gumerov Vadim Centre "Bioengineering”, Russian Academy of Sciences, Prosp. 60-let Oktyabrya, bld.7-1, 117312, Moscow, Russia aut Beletsky Alexey Centre "Bioengineering”, Russian Academy of Sciences, Prosp. 60-let Oktyabrya, bld.7-1, 117312, Moscow, Russia aut Perevalova Anna Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia aut Karpov Gennady Institute of Volcanology and Seismology, Russian Academy of Sciences, Petropavlovsk-Kamchatsky, Russia aut Bonch-Osmolovskaya Elizaveta Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia aut Ravin Nikolai Centre "Bioengineering”, Russian Academy of Sciences, Prosp. 60-let Oktyabrya, bld.7-1, 117312, Moscow, Russia aut Extremophiles Springer Japan 15/3(2011-05-01), 365-372 1431-0651 15:3<365 2011 15 792 https://doi.org/10.1007/s00792-011-0368-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0368-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mardanov Andrey Centre "Bioengineering”, Russian Academy of Sciences, Prosp. 60-let Oktyabrya, bld.7-1, 117312, Moscow, Russia aut NATIONALLICENCE 700 1- Gumerov Vadim Centre "Bioengineering”, Russian Academy of Sciences, Prosp. 60-let Oktyabrya, bld.7-1, 117312, Moscow, Russia aut NATIONALLICENCE 700 1- Beletsky Alexey Centre "Bioengineering”, Russian Academy of Sciences, Prosp. 60-let Oktyabrya, bld.7-1, 117312, Moscow, Russia aut NATIONALLICENCE 700 1- Perevalova Anna Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Karpov Gennady Institute of Volcanology and Seismology, Russian Academy of Sciences, Petropavlovsk-Kamchatsky, Russia aut NATIONALLICENCE 700 1- Bonch-Osmolovskaya Elizaveta Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Ravin Nikolai Centre "Bioengineering”, Russian Academy of Sciences, Prosp. 60-let Oktyabrya, bld.7-1, 117312, Moscow, Russia aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/3(2011-05-01), 365-372 1431-0651 15:3<365 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer