caa a22 4500 445818476 CHVBK 20180317145236.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00792-011-0371-6 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0371-6 Characterization of an OmpA-like outer membrane protein of the acidophilic iron-oxidizing bacterium, Acidithiobacillus ferrooxidans [Elektronische Daten] [Mohammed Manchur, Mei Kikumoto, Tadayoshi Kanao, Jun Takada, Kazuo Kamimura] An OmpA family protein (FopA) previously reported as one of the major outer membrane proteins of an acidophilic iron-oxidizing bacterium Acidithiobacillus ferrooxidans was characterized with emphasis on the modification by heat and the interaction with peptidoglycan. A 30-kDa band corresponding to the FopA protein was detected in outer membrane proteins extracted at 75°C or heated to 100°C for 10min prior to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). However, the band was not detected in outer membrane proteins extracted at ≤40°C and without boiling prior to electrophoresis. By Western blot analysis using the polyclonal antibody against the recombinant FopA, FopA was detected as bands with apparent molecular masses of 30 and 90kDa, suggesting that FopA existed as an oligomeric form in the outer membrane of A. ferrooxidans. Although the fopA gene with a sequence encoding the signal peptide was successfully expressed in the outer membrane of Escherichia coli, the recombinant FopA existed as a monomer in the outer membrane of E. coli. FopA was detected in peptidoglycan-associated proteins from A. ferrooxidans. The recombinant FopA also showed the peptidoglycan-binding activity. The Author(s), 2011 Acidithiobacillus ferrooxidans nationallicence Iron-oxidizing bacterium nationallicence Acidophile nationallicence Outer membrane protein nationallicence OmpA nationallicence Manchur Mohammed Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, 700-8530, Okayama, Japan aut Kikumoto Mei Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, 700-8530, Okayama, Japan aut Kanao Tadayoshi Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, 700-8530, Okayama, Japan aut Takada Jun Division of Chemical and Biological Technology, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, 700-8530, Okayama, Japan aut Kamimura Kazuo Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, 700-8530, Okayama, Japan aut Extremophiles Springer Japan 15/3(2011-05-01), 403-410 1431-0651 15:3<403 2011 15 792 https://doi.org/10.1007/s00792-011-0371-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0371-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Manchur Mohammed Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Kikumoto Mei Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Kanao Tadayoshi Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Takada Jun Division of Chemical and Biological Technology, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, 700-8530, Okayama, Japan aut NATIONALLICENCE 700 1- Kamimura Kazuo Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, 700-8530, Okayama, Japan aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/3(2011-05-01), 403-410 1431-0651 15:3<403 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer