caa a22 4500 445818484 CHVBK 20180317145236.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00792-011-0373-4 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0373-4 Biosynthesis of ω-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber [Elektronische Daten] [Lucie Siristova, Radek Luhovy, Karel Sigler, Tomas Rezanka] Two thermophilic strains belonging to Geobacillus stearothermophilus and Meiothermus ruber, which naturally do not synthesize ω-alicyclic fatty acids (ω-FAs) were cultivated with cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl carboxylic acids. Gas chromatography-mass spectrometry analysis of fatty acid methyl and picolinyl esters showed that both strains are able to synthesize ω-FAs when cultivated with the appropriate precursor. The incorporation of cyclic acids influenced the whole FA composition as well as membrane fluidity. Membrane fluidity of intact cells was studied by measuring the fluorescence polarisation of the probe l,6-diphenyl-1,3,5-hexatriene incorporated into membrane lipid bilayers. Cytoplasmic membrane became more fluid with increasing content of ω-FAs. This is caused by considerable changes in lipid packing within the membrane induced by the presence of ω-FAs not found in the natural environment of Geobacillus and Meiothermus strains. Springer, 2011 Thermophilic bacteria nationallicence Geobacillus nationallicence Meiothermus nationallicence ω-Alicyclic fatty acids nationallicence Membrane fluidity nationallicence Fluorescence anisotropy nationallicence Siristova Lucie Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology Prague, Technicka 3, 166 28, Prague, Czech Republic aut Luhovy Radek Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology Prague, Technicka 3, 166 28, Prague, Czech Republic aut Sigler Karel Institute of Microbiology, Videnska 1083, 142 20, Prague, Czech Republic aut Rezanka Tomas Institute of Microbiology, Videnska 1083, 142 20, Prague, Czech Republic aut Extremophiles Springer Japan 15/3(2011-05-01), 423-429 1431-0651 15:3<423 2011 15 792 https://doi.org/10.1007/s00792-011-0373-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0373-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Siristova Lucie Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology Prague, Technicka 3, 166 28, Prague, Czech Republic aut NATIONALLICENCE 700 1- Luhovy Radek Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology Prague, Technicka 3, 166 28, Prague, Czech Republic aut NATIONALLICENCE 700 1- Sigler Karel Institute of Microbiology, Videnska 1083, 142 20, Prague, Czech Republic aut NATIONALLICENCE 700 1- Rezanka Tomas Institute of Microbiology, Videnska 1083, 142 20, Prague, Czech Republic aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/3(2011-05-01), 423-429 1431-0651 15:3<423 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer