caa a22 4500 445818506 CHVBK 20180317145236.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00792-011-0362-7 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0362-7 Anaerobic transformation of carbon monoxide by microbial communities of Kamchatka hot springs [Elektronische Daten] [Tatiana Kochetkova, Igor Rusanov, Nikolay Pimenov, Tatyana Kolganova, Alexander Lebedinsky, Elizaveta Bonch-Osmolovskaya, Tatyana Sokolova] Carbon monoxide (CO) is one of the common gaseous compounds found in hot volcanic environments. It is known to serve as the growth substrate for a number of thermophilic prokaryotes, both aerobic and anaerobic. The goal of this work was to study the process of anaerobic transformation of CO by microbial communities inhabiting natural thermal environments: hot springs of Uzon Caldera, Kamchatka. The anaerobic microbial community of Treshchinny Spring (80°C, pH 6.5) was found to exhibit two peaks of affinity for CO (K S1=54nM and K S2=1μM). The actual rate of anaerobic CO transformation by the microbial community of this spring, calculated after obtaining the concentration dependence curve and extrapolated to the natural concentration of CO dissolved in the hot spring water (20nM), was found to be 120μmoll−1 of sediment day−1. In all the hot springs studied, more than 90% of the carbon of 14CO upon anaerobic incubation was recovered as 14CO2. From 1 to 5% of 14CO was transformed to volatile fatty acids (VFA). The number of microorganisms capable of anaerobic CO oxidation determined by dilution-to-extinction method reached 106cellsml−1 of sediment. CO-transforming anaerobic thermophilic microorganisms isolated from the springs under study exhibited hydrogenogenic type of CO oxidation and belonged to the bacterial genera Carboxydocella and Dictyoglomus. These data suggest a significant role of hydrogenogenic carboxydotrophic prokaryotes in anaerobic CO transformation in Uzon Caldera hot springs. Springer, 2011 Hydrothermal springs nationallicence Anaerobic CO oxidation nationallicence Thermophilic hydrogenogenic carboxydotrophic prokaryotes nationallicence Dictyoglomus nationallicence Carboxydocella nationallicence Kochetkova Tatiana Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-let Oktyabrya, 7/2, 117312, Moscow, Russia aut Rusanov Igor Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-let Oktyabrya, 7/2, 117312, Moscow, Russia aut Pimenov Nikolay Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-let Oktyabrya, 7/2, 117312, Moscow, Russia aut Kolganova Tatyana Bioengineering Center, Russian Academy of Sciences, Prospekt 60-let Oktyabrya, 7/1, 117312, Moscow, Russia aut Lebedinsky Alexander Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-let Oktyabrya, 7/2, 117312, Moscow, Russia aut Bonch-Osmolovskaya Elizaveta Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-let Oktyabrya, 7/2, 117312, Moscow, Russia aut Sokolova Tatyana Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-let Oktyabrya, 7/2, 117312, Moscow, Russia aut Extremophiles Springer Japan 15/3(2011-05-01), 319-325 1431-0651 15:3<319 2011 15 792 https://doi.org/10.1007/s00792-011-0362-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0362-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kochetkova Tatiana Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-let Oktyabrya, 7/2, 117312, Moscow, Russia aut NATIONALLICENCE 700 1- Rusanov Igor Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-let Oktyabrya, 7/2, 117312, Moscow, Russia aut NATIONALLICENCE 700 1- Pimenov Nikolay Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-let Oktyabrya, 7/2, 117312, Moscow, Russia aut NATIONALLICENCE 700 1- Kolganova Tatyana Bioengineering Center, Russian Academy of Sciences, Prospekt 60-let Oktyabrya, 7/1, 117312, Moscow, Russia aut NATIONALLICENCE 700 1- Lebedinsky Alexander Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-let Oktyabrya, 7/2, 117312, Moscow, Russia aut NATIONALLICENCE 700 1- Bonch-Osmolovskaya Elizaveta Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-let Oktyabrya, 7/2, 117312, Moscow, Russia aut NATIONALLICENCE 700 1- Sokolova Tatyana Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospekt 60-let Oktyabrya, 7/2, 117312, Moscow, Russia aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/3(2011-05-01), 319-325 1431-0651 15:3<319 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer