caa a22 4500 445818514 CHVBK 20180317145236.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00792-011-0366-3 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0366-3 Response to adverse conditions in two strains of the extremely halophilic species Salinibacter ruber [Elektronische Daten] [Jocelyn Brito-Echeverría, Marianna Lucio, Arantxa López-López, Josefa Antón, Philippe Schmitt-Kopplin, Ramón Rosselló-Móra] We have studied the response of the two closest relative strains M8 and M31 of Salinibacter ruber to environmental changes as the transition from exponential to stationary phase in a batch growth, and the submission to two different environmental stresses (dilution of the culture medium and temperature decrease). We monitored the changes in cultivability, ribosomal content by fluorescence in situ hybridization (FISH), and metabolic changes with high-field ion cyclotron Fourier transform mass spectrometry. In all cases, we could observe an important decrease in cultivability that was not accompanied by a decrease in FISH counts, pointing to a transition to viable but non-cultivable state rather than cell death. Furthermore, the metabolomic analyses indicated a common response of both strains to the different conditions assayed. Only a small portion of the detected masses could be annotated due to database constraints. Among them, the most remarkable changes could be attributed to modifications in the composition of the cell envelope, and especially in the cell membrane. We could track changes in the length or saturation of the fatty acids and in the composition of phospholipids involved in aminosugar, glycerolipid, and glycerophospholipid metabolic pathways. Springer, 2011 Adverse conditions nationallicence Extremely halophilic bacteria nationallicence Metabolomics nationallicence Brito-Echeverría Jocelyn Marine Microbiology Group, Institut Mediterrani d' Estudis Avançats (CSIC-UIB), 07190, Esporles, Spain aut Lucio Marianna Helmholtz Zentrum Muenchen, German Research Center for Environmental Health, Institute of Ecological Chemistry, Neuherberg, Germany aut López-López Arantxa Marine Microbiology Group, Institut Mediterrani d' Estudis Avançats (CSIC-UIB), 07190, Esporles, Spain aut Antón Josefa Departamento de Fisiología, Genética y Microbiología and Instituto Multidisciplinar de Estudios del Medio, Universidad de Alicante, Alicante, Spain aut Schmitt-Kopplin Philippe Helmholtz Zentrum Muenchen, German Research Center for Environmental Health, Institute of Ecological Chemistry, Neuherberg, Germany aut Rosselló-Móra Ramón Marine Microbiology Group, Institut Mediterrani d' Estudis Avançats (CSIC-UIB), 07190, Esporles, Spain aut Extremophiles Springer Japan 15/3(2011-05-01), 379-389 1431-0651 15:3<379 2011 15 792 https://doi.org/10.1007/s00792-011-0366-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0366-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Brito-Echeverría Jocelyn Marine Microbiology Group, Institut Mediterrani d' Estudis Avançats (CSIC-UIB), 07190, Esporles, Spain aut NATIONALLICENCE 700 1- Lucio Marianna Helmholtz Zentrum Muenchen, German Research Center for Environmental Health, Institute of Ecological Chemistry, Neuherberg, Germany aut NATIONALLICENCE 700 1- López-López Arantxa Marine Microbiology Group, Institut Mediterrani d' Estudis Avançats (CSIC-UIB), 07190, Esporles, Spain aut NATIONALLICENCE 700 1- Antón Josefa Departamento de Fisiología, Genética y Microbiología and Instituto Multidisciplinar de Estudios del Medio, Universidad de Alicante, Alicante, Spain aut NATIONALLICENCE 700 1- Schmitt-Kopplin Philippe Helmholtz Zentrum Muenchen, German Research Center for Environmental Health, Institute of Ecological Chemistry, Neuherberg, Germany aut NATIONALLICENCE 700 1- Rosselló-Móra Ramón Marine Microbiology Group, Institut Mediterrani d' Estudis Avançats (CSIC-UIB), 07190, Esporles, Spain aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/3(2011-05-01), 379-389 1431-0651 15:3<379 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer