caa a22 4500 445818530 CHVBK 20180317145236.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00792-011-0367-2 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0367-2 Conserved residues in membrane-bound acid pyrophosphatase from Sulfolobus tokodaii , a thermoacidophilic archaeon [Elektronische Daten] [Fumitoshi Manabe, Hirofumi Shoun, Takayoshi Wakagi] A membrane-intrinsic acid pyrophosphatase (ST2226) from Sulfolobus tokodaii, a thermoacidophilic archaeon, is possibly involved in glycoprotein biosynthesis and belongs to the phosphatidic acid phosphatase class 2 superfamily, including both membrane-intrinsic and soluble enzymes with divergent functions ranging from dephosphorylation of undecaprenylpyrophosphate and phospho-monoesters such as glucose-6-phosphate to vanadium-containing chloroperoxidation. ST2226 is an archaeal ortholog of these enzymes sharing a common phosphatase motif. Through site-directed mutagenesis as to each of the conserved residues, the catalytic roles of the latter were deduced, as well as the transmembrane topology with all the conserved residues in close proximity to the outside of the membrane. Springer, 2011 Phosphatase motif nationallicence Pyrophosphatase nationallicence Dolicholpyrophosphatase nationallicence Membrane integral enzyme nationallicence Archaea nationallicence Manabe Fumitoshi Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut Shoun Hirofumi Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut Wakagi Takayoshi Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut Extremophiles Springer Japan 15/3(2011-05-01), 359-364 1431-0651 15:3<359 2011 15 792 https://doi.org/10.1007/s00792-011-0367-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0367-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Manabe Fumitoshi Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut NATIONALLICENCE 700 1- Shoun Hirofumi Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut NATIONALLICENCE 700 1- Wakagi Takayoshi Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, 113-8657, Tokyo, Japan aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/3(2011-05-01), 359-364 1431-0651 15:3<359 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer