caa a22 4500 445818549 CHVBK 20180317145236.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00792-011-0365-4 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0365-4 Biochemical characterization of glyceraldehyde-3-phosphate dehydrogenase from Thermococcus kodakarensis KOD1 [Elektronische Daten] [Baolei Jia, Le Linh, Sangmin Lee, Bang Pham, Jinliang Liu, Hongyu Pan, Shihong Zhang, Gang-Won Cheong] Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) plays an essential role in glycolysis by catalyzing the conversion of d-glyceraldehyde 3-phosphate (d-G3P) to 1,3-diphosphoglycerate using NAD+ as a cofactor. In this report, the GAPDH gene from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1 (GAPDH-tk) was cloned and the protein was purified to homogeneity. GAPDH-tk exists as a homotetramer with a native molecular mass of 145kDa; the subunit molecular mass was 37kDa. GAPDH-tk is a thermostable protein with a half-life of 5h at 80-90°C. The apparent K m values for NAD+ and d-G3P were 77.8±7.5μM and 49.3±3.0μM, respectively, with V max values of 45.1±0.8U/mg and 59.6±1.3U/mg, respectively. Transmission electron microscopy (TEM) and image processing confirmed that GAPDH-tk has a tetrameric structure. Interestingly, GAPDH-tk migrates as high molecular mass forms (~232kDa and ~669kDa) in response to oxidative stress. Springer, 2011 GAPDH nationallicence Thermophilic protein nationallicence Oxidative stress nationallicence Protein aggregation nationallicence TEM nationallicence Jia Baolei College of Plant Sciences, Jilin University, 130-062, Changchun, China aut Linh Le Division of Applied Life Sciences (BK21 Program), Gyeongsang National University, 660-701, Jinju, Korea aut Lee Sangmin Division of Applied Life Sciences (BK21 Program), Gyeongsang National University, 660-701, Jinju, Korea aut Pham Bang Division of Applied Life Sciences (BK21 Program), Gyeongsang National University, 660-701, Jinju, Korea aut Liu Jinliang College of Plant Sciences, Jilin University, 130-062, Changchun, China aut Pan Hongyu College of Plant Sciences, Jilin University, 130-062, Changchun, China aut Zhang Shihong College of Plant Sciences, Jilin University, 130-062, Changchun, China aut Cheong Gang-Won Division of Applied Life Sciences (BK21 Program), Gyeongsang National University, 660-701, Jinju, Korea aut Extremophiles Springer Japan 15/3(2011-05-01), 337-346 1431-0651 15:3<337 2011 15 792 https://doi.org/10.1007/s00792-011-0365-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0365-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jia Baolei College of Plant Sciences, Jilin University, 130-062, Changchun, China aut NATIONALLICENCE 700 1- Linh Le Division of Applied Life Sciences (BK21 Program), Gyeongsang National University, 660-701, Jinju, Korea aut NATIONALLICENCE 700 1- Lee Sangmin Division of Applied Life Sciences (BK21 Program), Gyeongsang National University, 660-701, Jinju, Korea aut NATIONALLICENCE 700 1- Pham Bang Division of Applied Life Sciences (BK21 Program), Gyeongsang National University, 660-701, Jinju, Korea aut NATIONALLICENCE 700 1- Liu Jinliang College of Plant Sciences, Jilin University, 130-062, Changchun, China aut NATIONALLICENCE 700 1- Pan Hongyu College of Plant Sciences, Jilin University, 130-062, Changchun, China aut NATIONALLICENCE 700 1- Zhang Shihong College of Plant Sciences, Jilin University, 130-062, Changchun, China aut NATIONALLICENCE 700 1- Cheong Gang-Won Division of Applied Life Sciences (BK21 Program), Gyeongsang National University, 660-701, Jinju, Korea aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/3(2011-05-01), 337-346 1431-0651 15:3<337 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer