caa a22 4500 445818565 CHVBK 20180317145236.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00792-011-0377-0 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0377-0 Gluconeotrehalose is the principal organic solute in the psychrotolerant bacterium Carnobacterium strain 17-4 [Elektronische Daten] [Pedro Lamosa, Ana Mingote, Tatiana Groudieva, Barbara Klippel, Ksenia Egorova, Dina Jabbour, Helena Santos, Garabed Antranikian] A high proportion of microorganisms that colonise cold environments originate from marine sites; hence, they must combine adaptation to low temperature with osmoregulation. However, little or nothing is known about the nature of compatible solutes used by cold-adapted organisms to balance the osmotic pressure of the external medium. We studied the intracellular accumulation of small organic solutes in the Arctic isolate Carnobacterium strain 17-4 as a function of the growth temperature and the NaCl concentration in the medium. Data on 16S rDNA sequence and DNA-DNA hybridisation tests corroborate the assignment of this isolate as a new species of the bacterial genus Carnobacterium. The growth profiles displayed maximal specific growth rate at 30°C in medium without NaCl, and maximal values of final biomass at growth temperatures between 10 and 20°C. Therefore, Carnobacterium strain 17-4 exhibits halotolerant and psychrotolerant behaviours. The solute pool contained glycine-betaine, the main solute used for osmoregulation, and an unknown compound whose structure was identified as α-glucopyranosyl-(1-3)-β-glucopyranosyl-(1-1)-α-glucopyranose (abbreviated as gluconeotrehalose), using nuclear magnetic resonance and mass spectrometry. This unusual solute consistently accumulated to high levels (0.35±0.05mg/mg cell protein) regardless of the growth temperature or salinity. The efficiency of gluconeotrehalose in the stabilisation of four model enzymes against heat damage was also assessed, and the effects were highly protein dependent. The lack of variation in the gluconeotrehalose content observed under heat stress, osmotic stress, and starvation provides no clue for the physiological role of this rare solute. Springer, 2011 Cold-adapted bacterium nationallicence Osmoregulation nationallicence α,β-Trehalose nationallicence Enzyme stabilisation nationallicence Trisaccharide nationallicence Lamosa Pedro Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República-EAN, Apartado 127, 2780-157, Oeiras, Portugal aut Mingote Ana Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República-EAN, Apartado 127, 2780-157, Oeiras, Portugal aut Groudieva Tatiana Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstr 12, 21073, Hamburg, Germany aut Klippel Barbara Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstr 12, 21073, Hamburg, Germany aut Egorova Ksenia Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstr 12, 21073, Hamburg, Germany aut Jabbour Dina Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstr 12, 21073, Hamburg, Germany aut Santos Helena Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República-EAN, Apartado 127, 2780-157, Oeiras, Portugal aut Antranikian Garabed Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstr 12, 21073, Hamburg, Germany aut Extremophiles Springer Japan 15/4(2011-07-01), 463-472 1431-0651 15:4<463 2011 15 792 https://doi.org/10.1007/s00792-011-0377-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0377-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lamosa Pedro Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República-EAN, Apartado 127, 2780-157, Oeiras, Portugal aut NATIONALLICENCE 700 1- Mingote Ana Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República-EAN, Apartado 127, 2780-157, Oeiras, Portugal aut NATIONALLICENCE 700 1- Groudieva Tatiana Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstr 12, 21073, Hamburg, Germany aut NATIONALLICENCE 700 1- Klippel Barbara Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstr 12, 21073, Hamburg, Germany aut NATIONALLICENCE 700 1- Egorova Ksenia Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstr 12, 21073, Hamburg, Germany aut NATIONALLICENCE 700 1- Jabbour Dina Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstr 12, 21073, Hamburg, Germany aut NATIONALLICENCE 700 1- Santos Helena Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República-EAN, Apartado 127, 2780-157, Oeiras, Portugal aut NATIONALLICENCE 700 1- Antranikian Garabed Institute of Technical Microbiology, Hamburg University of Technology, Kasernenstr 12, 21073, Hamburg, Germany aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/4(2011-07-01), 463-472 1431-0651 15:4<463 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer