caa a22 4500 445818573 CHVBK 20180317145236.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00792-011-0379-y doi (NATIONALLICENCE)springer-10.1007/s00792-011-0379-y Genomic analysis of Acidianus hospitalis W1 a host for studying crenarchaeal virus and plasmid life cycles [Elektronische Daten] [Xiao-Yan You, Chao Liu, Sheng-Yue Wang, Cheng-Ying Jiang, Shiraz Shah, David Prangishvili, Qunxin She, Shuang-Jiang Liu, Roger Garrett] The Acidianus hospitalis W1 genome consists of a minimally sized chromosome of about 2.13Mb and a conjugative plasmid pAH1 and it is a host for the model filamentous lipothrixvirus AFV1. The chromosome carries three putative replication origins in conserved genomic regions and two large regions where non-essential genes are clustered. Within these variable regions, a few orphan orfB and other elements of the IS200/607/605 family are concentrated with a novel class of MITE-like repeat elements. There are also 26 highly diverse vapBC antitoxin-toxin gene pairs proposed to facilitate maintenance of local chromosomal regions and to minimise the impact of environmental stress. Complex and partially defective CRISPR/Cas/Cmr immune systems are present and interspersed with five vapBC gene pairs. Remnants of integrated viral genomes and plasmids are located at five intron-less tRNA genes and several non-coding RNA genes are predicted that are conserved in other Sulfolobus genomes. The putative metabolic pathways for sulphur metabolism show some significant differences from those proposed for other Acidianus and Sulfolobus species. The small and relatively stable genome of A. hospitalis W1 renders it a promising candidate for developing the first Acidianus genetic systems. The Author(s), 2011 Toxin-antitoxin VapBC nationallicence CRISPR nationallicence Sulphur metabolism nationallicence O rfB element nationallicence MITE nationallicence You Xiao-Yan State Key Laboratory of Microbial Resources and Center for Environmental Microbiology, Institute of Microbiology, Chinese Academy of Sciences, Bei-Chen-Xi-Lu No. 1 Chao-Yang District, 100101, Beijing, People's Republic of China aut Liu Chao Archaea Centre, Department of Biology, Copenhagen University, Ole Maaløes Vej 5, 2200, N Copenhagen, Denmark aut Wang Sheng-Yue Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center, Shanghai, People's Republic of China aut Jiang Cheng-Ying State Key Laboratory of Microbial Resources and Center for Environmental Microbiology, Institute of Microbiology, Chinese Academy of Sciences, Bei-Chen-Xi-Lu No. 1 Chao-Yang District, 100101, Beijing, People's Republic of China aut Shah Shiraz Archaea Centre, Department of Biology, Copenhagen University, Ole Maaløes Vej 5, 2200, N Copenhagen, Denmark aut Prangishvili David Molecular Biology of the Gene in Extremophiles Unit, Institut Pasteur, rue Dr Roux 25, 75724, Paris Cedex, France aut She Qunxin Archaea Centre, Department of Biology, Copenhagen University, Ole Maaløes Vej 5, 2200, N Copenhagen, Denmark aut Liu Shuang-Jiang State Key Laboratory of Microbial Resources and Center for Environmental Microbiology, Institute of Microbiology, Chinese Academy of Sciences, Bei-Chen-Xi-Lu No. 1 Chao-Yang District, 100101, Beijing, People's Republic of China aut Garrett Roger Archaea Centre, Department of Biology, Copenhagen University, Ole Maaløes Vej 5, 2200, N Copenhagen, Denmark aut Extremophiles Springer Japan 15/4(2011-07-01), 487-497 1431-0651 15:4<487 2011 15 792 https://doi.org/10.1007/s00792-011-0379-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0379-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- You Xiao-Yan State Key Laboratory of Microbial Resources and Center for Environmental Microbiology, Institute of Microbiology, Chinese Academy of Sciences, Bei-Chen-Xi-Lu No. 1 Chao-Yang District, 100101, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Liu Chao Archaea Centre, Department of Biology, Copenhagen University, Ole Maaløes Vej 5, 2200, N Copenhagen, Denmark aut NATIONALLICENCE 700 1- Wang Sheng-Yue Shanghai-MOST Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Cheng-Ying State Key Laboratory of Microbial Resources and Center for Environmental Microbiology, Institute of Microbiology, Chinese Academy of Sciences, Bei-Chen-Xi-Lu No. 1 Chao-Yang District, 100101, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Shah Shiraz Archaea Centre, Department of Biology, Copenhagen University, Ole Maaløes Vej 5, 2200, N Copenhagen, Denmark aut NATIONALLICENCE 700 1- Prangishvili David Molecular Biology of the Gene in Extremophiles Unit, Institut Pasteur, rue Dr Roux 25, 75724, Paris Cedex, France aut NATIONALLICENCE 700 1- She Qunxin Archaea Centre, Department of Biology, Copenhagen University, Ole Maaløes Vej 5, 2200, N Copenhagen, Denmark aut NATIONALLICENCE 700 1- Liu Shuang-Jiang State Key Laboratory of Microbial Resources and Center for Environmental Microbiology, Institute of Microbiology, Chinese Academy of Sciences, Bei-Chen-Xi-Lu No. 1 Chao-Yang District, 100101, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Garrett Roger Archaea Centre, Department of Biology, Copenhagen University, Ole Maaløes Vej 5, 2200, N Copenhagen, Denmark aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/4(2011-07-01), 487-497 1431-0651 15:4<487 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer