caa a22 4500 44581859X CHVBK 20180317145236.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00792-011-0382-3 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0382-3 The major lipid cores of the archaeon Ignisphaera aggregans [Elektronische Daten] implications for the phylogeny and biosynthesis of glycerol monoalkyl glycerol tetraether isoprenoid lipids [Chris Knappy, Charlotte Nunn, Hugh Morgan, Brendan Keely] The lipid cores from Ignisphaera aggregans, a hyperthermophilic Crenarchaeon recently isolated from New Zealand hot springs, have been profiled by liquid chromatography-tandem mass spectrometry. The distribution revealed includes relatively high proportions of monoalkyl (also known as H-shaped) tetraether cores which have previously been implicated as kingdom-specific biomarkers for the Euryarchaeota. Such high expression of monoalkyl tetraether lipids is unusual in the archaeal domain and may indicate that formation of these components is an adaptive mechanism that allows I. aggregans to regulate membrane behaviour at high temperatures. The observed dialkyl tetraether and monoalkyl tetraether lipid distributions are similar but not fully concordant, showing differences in the average number of incorporated rings. The similarity supports a biosynthetic route to the ring-containing dialkyl and monoalkyl tetraether lipids via a dialkyl tetraether core containing zero rings, or a closely related structural relative, as an intermediate. Currently, however, the precise nature of the biosynthetic route to these lipids cannot be deduced. Springer, 2011 Ignisphaera nationallicence Archaea nationallicence Tetraether lipids nationallicence H-shaped lipids nationallicence Isoprenoids nationallicence Tandem mass spectrometry nationallicence CID : Collision-induced dissociation nationallicence Cp : Cyclopentyl nationallicence Da : Daltons nationallicence DCM : Dichloromethane nationallicence GDD : Glycerol dialkyl diether nationallicence GDGT : Glycerol dialkyl glycerol tetraether nationallicence GMD : Glycerol monoalkyl diether nationallicence GMGT : Glycerol monoalkyl glycerol tetraether nationallicence GTGT : Glycerol trialkyl glycerol tetraether nationallicence LC-MS/MS : Liquid chromatography-tandem mass spectrometry nationallicence [M+H]+ : Protonated molecule nationallicence APCI : Atmospheric pressure chemical ionisation nationallicence ESI : Electrospray ionisation nationallicence Knappy Chris Department of Chemistry, University of York, YO10 5DD, York, UK aut Nunn Charlotte Centre for Extremophile Research, University of Bath, BA2 7AY, Bath, UK aut Morgan Hugh Thermophile Research Unit, University of Waikato, Hamilton, New Zealand aut Keely Brendan Department of Chemistry, University of York, YO10 5DD, York, UK aut Extremophiles Springer Japan 15/4(2011-07-01), 517-528 1431-0651 15:4<517 2011 15 792 https://doi.org/10.1007/s00792-011-0382-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0382-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Knappy Chris Department of Chemistry, University of York, YO10 5DD, York, UK aut NATIONALLICENCE 700 1- Nunn Charlotte Centre for Extremophile Research, University of Bath, BA2 7AY, Bath, UK aut NATIONALLICENCE 700 1- Morgan Hugh Thermophile Research Unit, University of Waikato, Hamilton, New Zealand aut NATIONALLICENCE 700 1- Keely Brendan Department of Chemistry, University of York, YO10 5DD, York, UK aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/4(2011-07-01), 517-528 1431-0651 15:4<517 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer