caa a22 4500 445818603 CHVBK 20180317145236.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00792-011-0380-5 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0380-5 Regulation of expression of trehalose-6-phosphate synthase during cold shock in Arthrobacter strain A3 [Elektronische Daten] [Xi-Ming Chen, Ying Jiang, Yuan-Ting Li, Hai-Hong Zhang, Jie Li, Xing Chen, Qi Zhao, Jing Zhao, Jing Si, Zhi-Wei Lin, Hua Zhang, Paul Dyson, Li-Zhe An] Trehalose is a chemical chaperone known to protect a variety of organisms against cold stress. Members of the genus Arthrobacter, which belongs to the Actinomycetales group, exhibit strong resistance to stress conditions, but exactly how trehalose synthesis is regulated in conditions of cold stress is still unknown. Here, we report that Arthrobacter strain A3, which was isolated from the alpine permafrost, has only two trehalose synthesis pathways (OtsA/B and TreS), while other Arthrobacter spp. have three. Mutants and immunoblot analyses indicate that trehalose is mainly synthesized via OtsA at low temperatures in Arthrobacter strain A3. Therefore, we have focused on the regulation of OtsA expression during cold shock. The results indicated that both low temperature and accumulation of trehalose can inhibit OtsA expression. The elongation factor Tu, which binds to OtsA, stabilizes the expression of OtsA in the cold. Springer, 2011 Trehalose nationallicence OtsA nationallicence Arthrobacter nationallicence Cold shock nationallicence EF-Tu nationallicence Chen Xi-Ming State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Jiang Ying State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Li Yuan-Ting State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Zhang Hai-Hong State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Li Jie State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Chen Xing Institute of Cancer Biology and Drug Screening, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Zhao Qi State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Zhao Jing State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Si Jing State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Lin Zhi-Wei Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Animal Science College, Zhejiang University, 310029, Hangzhou, People's Republic of China aut Zhang Hua State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Dyson Paul Institute of Life Science, School of Medicine, Swansea University, Singleton Park, SA2 8PP, Swansea, UK aut An Li-Zhe State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut Extremophiles Springer Japan 15/4(2011-07-01), 499-508 1431-0651 15:4<499 2011 15 792 https://doi.org/10.1007/s00792-011-0380-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0380-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Xi-Ming State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Ying State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Li Yuan-Ting State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Hai-Hong State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Li Jie State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Chen Xing Institute of Cancer Biology and Drug Screening, School of Life Sciences, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Qi State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Jing State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Si Jing State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Lin Zhi-Wei Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Animal Science College, Zhejiang University, 310029, Hangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Hua State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 700 1- Dyson Paul Institute of Life Science, School of Medicine, Swansea University, Singleton Park, SA2 8PP, Swansea, UK aut NATIONALLICENCE 700 1- An Li-Zhe State Key Laboratory of Arid and Grassland Agroecology of Ministry of Education, Lanzhou University, 730000, Lanzhou, People's Republic of China aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/4(2011-07-01), 499-508 1431-0651 15:4<499 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer