caa a22 4500 445818611 CHVBK 20180317145236.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00792-011-0383-2 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0383-2 The GINS complex from the thermophilic archaeon, Thermoplasma acidophilum may function as a homotetramer in DNA replication [Elektronische Daten] [Hiromi Ogino, Sonoko Ishino, Kouta Mayanagi, Gyri Haugland, Nils-Kåre Birkeland, Akihiko Yamagishi, Yoshizumi Ishino] The eukaryotic GINS heterotetramer, consisting of Sld5, Psf1, Psf2, and Psf3, participates in "CMG complex” formation with mini-chromosome maintenance (MCM) and Cdc45 as a key component of a replicative helicase. There are only two homologs of the GINS proteins in Archaea, and these proteins, Gins51 and Gins23, form a heterotetrameric GINS with a 2:2 molar ratio. The Pyrococcus furiosus GINS stimulates the ATPase and helicase activities of its cognate MCM, whereas the Sulfolobus solfataricus GINS does not affect those activities of its cognate MCM, although the proteins bind each other. Intriguingly, Thermoplasma acidophilum, as well as many euryarchaea, have only one gene encoding the sequence homologous to that of archaeal Gins protein (Gins51) on the genome. In this study, we investigated the biochemical properties of the gene product (TaGins51). A gel filtration and electron microscopy revealed that TaGins51 forms a homotetramer. A physical interaction between TaGins51 and TaMcm was detected by a surface plasmon resonance analysis. Unexpectedly, TaGins51 inhibited the ATPase activity, but did not affect the helicase activity of its cognate MCM. These results suggest that another factor is required to form a stable helicase complex with MCM and GINS at the replication fork in T. acidophilum cells. Springer, 2011 Archaea nationallicence DNA replication nationallicence Replisome nationallicence DNA helicase nationallicence Protein interaction nationallicence Thermoacidophile nationallicence Ogino Hiromi Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Fukuoka, Japan aut Ishino Sonoko Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Fukuoka, Japan aut Mayanagi Kouta Medical Institute of Bioregulation, Kyushu University, 812-8582, Fukuoka, Japan aut Haugland Gyri Department of Biology, University of Bergen, Bergen, Norway aut Birkeland Nils-Kåre Department of Biology and Centre for Geobiology, University of Bergen, Bergen, Norway aut Yamagishi Akihiko Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan aut Ishino Yoshizumi Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Fukuoka, Japan aut Extremophiles Springer Japan 15/4(2011-07-01), 529-539 1431-0651 15:4<529 2011 15 792 https://doi.org/10.1007/s00792-011-0383-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0383-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ogino Hiromi Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Fukuoka, Japan aut NATIONALLICENCE 700 1- Ishino Sonoko Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Fukuoka, Japan aut NATIONALLICENCE 700 1- Mayanagi Kouta Medical Institute of Bioregulation, Kyushu University, 812-8582, Fukuoka, Japan aut NATIONALLICENCE 700 1- Haugland Gyri Department of Biology, University of Bergen, Bergen, Norway aut NATIONALLICENCE 700 1- Birkeland Nils-Kåre Department of Biology and Centre for Geobiology, University of Bergen, Bergen, Norway aut NATIONALLICENCE 700 1- Yamagishi Akihiko Department of Molecular Biology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan aut NATIONALLICENCE 700 1- Ishino Yoshizumi Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, 812-8581, Fukuoka, Fukuoka, Japan aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/4(2011-07-01), 529-539 1431-0651 15:4<529 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer