caa a22 4500 44581862X CHVBK 20180317145236.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00792-011-0378-z doi (NATIONALLICENCE)springer-10.1007/s00792-011-0378-z Bacterial diversity in five Icelandic geothermal waters: temperature and sinter growth rate effects [Elektronische Daten] [Dominique Tobler, Liane Benning] The microbial ecology associated with siliceous sinters was studied in five geochemically diverse Icelandic geothermal systems. Bacterial 16S rRNA clone libraries were constructed from water-saturated precipitates from each site resulting in a total of 342 bacterial clone sequences and 43 species level phylotypes. In near-neutral, saline (2.6-4.7% salinity) geothermal waters where sinter growth varied between 10 and ~300kgyear−1m−2, 16S rRNA gene analyses revealed very low (no OTUs could be detected) to medium (9OTUs) microbial activity. The most dominant phylotypes found in these waters belong to marine genera of the Proteobacteria. In contrast, in alkaline (pH=9-10), meteoric geothermal waters with temperature=66-96°C and <1-20kgyear−1m−2 sinter growth, extensive biofilms (a total of 34 OTUs) were observed within the waters and these were dominated by members of the class Aquificae (mostly related to Thermocrinis), Deinococci (Thermus species) as well as Proteobacteria. The observed phylogenetic diversity (i.e., number and composition of detected OTUs) is argued to be related to the physico-chemical regime prevalent in the studied geothermal waters; alkaliphilic thermophilic microbial communities with phylotypes related to heterotrophic and autotrophic microorganisms developed in alkaline high temperature waters, whereas halophilic mesophilic communities dominated coastal geothermal waters. Springer, 2011 Bacterial diversity nationallicence Hot spring nationallicence Phylogeny nationallicence Thermophiles nationallicence Ecology nationallicence Geothermal water nationallicence Silica sinter nationallicence Tobler Dominique Earth and Biosphere Institute, School of Earth and Environment, University of Leeds, LS2 9JT, Leeds, UK aut Benning Liane Earth and Biosphere Institute, School of Earth and Environment, University of Leeds, LS2 9JT, Leeds, UK aut Extremophiles Springer Japan 15/4(2011-07-01), 473-485 1431-0651 15:4<473 2011 15 792 https://doi.org/10.1007/s00792-011-0378-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0378-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tobler Dominique Earth and Biosphere Institute, School of Earth and Environment, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 700 1- Benning Liane Earth and Biosphere Institute, School of Earth and Environment, University of Leeds, LS2 9JT, Leeds, UK aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/4(2011-07-01), 473-485 1431-0651 15:4<473 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer