caa a22 4500 445818646 CHVBK 20180317145237.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00792-011-0389-9 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0389-9 The relationship between a coiled morphology and Mbl in alkaliphilic Bacillus halodurans C-125 at neutral pH values [Elektronische Daten] [Shun Fujinami, Takako Sato, Masahiro Ito] The facultative alkaliphilic Bacillus halodurans C-125 can grow in a pH range from 6.8 to 10.8. The morphology of the cells grown at pH values above 7.5 is rod shaped, whereas, that gown at pH values less than 7.5 is coiled. Cytoplasmic membrane staining revealed that this coiled morphology was formed not by one filamentous cell, but by many chained bent/non-bent cells. Prokaryotic actin and tubulin homologs (MreB, Mbl MreBH, and FtsZ, respectively) are known to function as bacterial cytoskeleton proteins. The transcription levels of ftsZ, mreB, and mreBH genes were hardly affected by growth pH. However, the level of the mbl gene was significantly decreased at neutral pH values. Moreover, the expression level of the Mbl protein at pH 7.0 was about one-fourth of that at pH 10. Immunofluorescence microscopy (IFM) showed that the Mbl protein was localized as a helical structure in the rod-shaped cell grown at pH 10, whereas a helical structure was not observed in the cells grown at pH 7.0. Fluorescent vancomycin staining showed insertion of new peptidoglycan strands of sidewalls occurred in the cells grown at pH 7.0. These data suggested that a decrease in the expression level of the Mbl protein can influence the morphology of B. halodurans C-125 grown at pH 7.0 without influencing insertion of new peptidoglycan strands. Springer, 2011 Alkaliphiles nationallicence Bacillus halodurans C-125 nationallicence Bacterial cytoskeleton nationallicence Morphology nationallicence IFM : Immunofluorescence microscopy nationallicence Fujinami Shun Bio-Nano Electronics Research Centre, Toyo University, 2100 Kujirai, 350-8585, Kawagoe, Saitama, Japan aut Sato Takako Marine Biodiversity Research Program, Institute of Biogeosciences (Biogeos), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, 237-0061, Yokosuka, Kanagawa, Japan aut Ito Masahiro Bio-Nano Electronics Research Centre, Toyo University, 2100 Kujirai, 350-8585, Kawagoe, Saitama, Japan aut Extremophiles Springer Japan 15/5(2011-09-01), 587-596 1431-0651 15:5<587 2011 15 792 https://doi.org/10.1007/s00792-011-0389-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0389-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fujinami Shun Bio-Nano Electronics Research Centre, Toyo University, 2100 Kujirai, 350-8585, Kawagoe, Saitama, Japan aut NATIONALLICENCE 700 1- Sato Takako Marine Biodiversity Research Program, Institute of Biogeosciences (Biogeos), Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, 237-0061, Yokosuka, Kanagawa, Japan aut NATIONALLICENCE 700 1- Ito Masahiro Bio-Nano Electronics Research Centre, Toyo University, 2100 Kujirai, 350-8585, Kawagoe, Saitama, Japan aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/5(2011-09-01), 587-596 1431-0651 15:5<587 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer