caa a22 4500 445818662 CHVBK 20180317145237.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00792-011-0387-y doi (NATIONALLICENCE)springer-10.1007/s00792-011-0387-y Characterization of Halanaerocella petrolearia gen. nov., sp. nov., a new anaerobic moderately halophilic fermentative bacterium isolated from a deep subsurface hypersaline oil reservoir [Elektronische Daten] New taxa: Firmicutes (Class Clostridia , Order Halanaerobiales , Halobacteroidaceae , Halobacteroides ) [G. Gales, N. Chehider, C. Joulian, F. Battaglia-Brunet, J.-L. Cayol, A. Postec, J. Borgomano, I. Neria-Gonzalez, B. Lomans, B. Ollivier, D. Alazard] An anaerobic, halophilic, and fermentative bacterium, strain S200T, was isolated from a core sample of a deep hypersaline oil reservoir. Cells were rod-shaped, non-motile, and stained Gram-positive. It grew at NaCl concentrations ranging from 6 to 26% (w/v), with optimal growth at 15% (w/v) NaCl, and at temperatures between 25 and 47°C with an optimum at 40-45°C. The optimum pH was 7.3 (range 6.2-8.8; no growth at pH 5.8 and pH 9). The doubling time in optimized growth conditions was 3.5h. Strain S200T used exclusively carbohydrates as carbon and energy sources. The end products of glucose degradation were lactate, formate, ethanol, acetate, H2, and CO2. The predominant cellular fatty acids were non-branched fatty acids C16:1, C16:0, and C14:0. The G+C mole% of the DNA was 32.7%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain S200T formed a distinct lineage within the family Halobacteroidaceae, order Halanaerobiales, and was most closely related to Halanaerobaculum tunisiense DSM 19997T and Halobacteroides halobius DSM 5150T, with sequence similarity of 92.3 and 91.9%, respectively. On the basis of its physiological and genotypic properties, strain S200T is proposed to be assigned to a novel species of a novel genus, for which the name Halanaerocella petrolearia is proposed. The type strain of Halanaerocella petrolearia is strain S200T (=DSM 22693T=JCM 16358T). Springer, 2011 Anaerobic nationallicence Halophilic nationallicence Taxonomy nationallicence Halanaerocella petrolearia nationallicence Oil reservoir nationallicence Gales G. Université de Provence, Laboratoire de Géologie des Systèmes et des Réservoirs Carbonatés, EA 4234, case 67, 3 Place Victor Hugo, 13331, Marseille Cedex 03, France aut Chehider N. Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France aut Joulian C. BRGM, Unité Ecotechnologies, 3 Avenue Claude Guillemin, BP 36009, 45060, Orléans Cedex 2, France aut Battaglia-Brunet F. BRGM, Unité Ecotechnologies, 3 Avenue Claude Guillemin, BP 36009, 45060, Orléans Cedex 2, France aut Cayol J.-L Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France aut Postec A. Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France aut Borgomano J. Université de Provence, Laboratoire de Géologie des Systèmes et des Réservoirs Carbonatés, EA 4234, case 67, 3 Place Victor Hugo, 13331, Marseille Cedex 03, France aut Neria-Gonzalez I. Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France aut Lomans B. Emerging Technologies—Subsurface, Projects & Technologies, Shell International Exploration and Production B.V, Kessler Park 1, 2288 GS, Rijswijk, The Netherlands aut Ollivier B. Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France aut Alazard D. Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France aut Extremophiles Springer Japan 15/5(2011-09-01), 565-571 1431-0651 15:5<565 2011 15 792 https://doi.org/10.1007/s00792-011-0387-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0387-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gales G. Université de Provence, Laboratoire de Géologie des Systèmes et des Réservoirs Carbonatés, EA 4234, case 67, 3 Place Victor Hugo, 13331, Marseille Cedex 03, France aut NATIONALLICENCE 700 1- Chehider N. Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France aut NATIONALLICENCE 700 1- Joulian C. BRGM, Unité Ecotechnologies, 3 Avenue Claude Guillemin, BP 36009, 45060, Orléans Cedex 2, France aut NATIONALLICENCE 700 1- Battaglia-Brunet F. BRGM, Unité Ecotechnologies, 3 Avenue Claude Guillemin, BP 36009, 45060, Orléans Cedex 2, France aut NATIONALLICENCE 700 1- Cayol J.-L Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France aut NATIONALLICENCE 700 1- Postec A. Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France aut NATIONALLICENCE 700 1- Borgomano J. Université de Provence, Laboratoire de Géologie des Systèmes et des Réservoirs Carbonatés, EA 4234, case 67, 3 Place Victor Hugo, 13331, Marseille Cedex 03, France aut NATIONALLICENCE 700 1- Neria-Gonzalez I. Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France aut NATIONALLICENCE 700 1- Lomans B. Emerging Technologies—Subsurface, Projects & Technologies, Shell International Exploration and Production B.V, Kessler Park 1, 2288 GS, Rijswijk, The Netherlands aut NATIONALLICENCE 700 1- Ollivier B. Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France aut NATIONALLICENCE 700 1- Alazard D. Laboratoire de Microbiologie IRD, UMR 180, Universités de Provence et de la Méditerranée, ESIL, case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/5(2011-09-01), 565-571 1431-0651 15:5<565 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer