caa a22 4500 445818670 CHVBK 20180317145237.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00792-011-0386-z doi (NATIONALLICENCE)springer-10.1007/s00792-011-0386-z Archaeal and bacterial diversity in hot springs on the Tibetan Plateau, China [Elektronische Daten] [Qiuyuan Huang, Christina Dong, Raymond Dong, Hongchen Jiang, Shang Wang, Genhou Wang, Bin Fang, Xiaoxue Ding, Lu Niu, Xin Li, Chuanlun Zhang, Hailiang Dong] The diversity of archaea and bacteria was investigated in ten hot springs (elevation >4600m above sea level) in Central and Central-Eastern Tibet using 16S rRNA gene phylogenetic analysis. The temperature and pH of these hot springs were 26-81°C and close to neutral, respectively. A total of 959 (415 and 544 for bacteria and archaea, respectively) clone sequences were obtained. Phylogenetic analysis showed that bacteria were more diverse than archaea and that these clone sequences were classified into 82 bacterial and 41 archaeal operational taxonomic units (OTUs), respectively. The retrieved bacterial clones were mainly affiliated with four known groups (i.e., Firmicutes, Proteobacteria, Cyanobacteria, Chloroflexi), which were similar to those in other neutral-pH hot springs at low elevations. In contrast, most of the archaeal clones from the Tibetan hot springs were affiliated with Thaumarchaeota, a newly proposed archaeal phylum. The dominance of Thaumarchaeota in the archaeal community of the Tibetan hot springs appears to be unique, although the exact reasons are not yet known. Statistical analysis showed that diversity indices of both archaea and bacteria were not statistically correlated with temperature, which is consistent with previous studies. Springer, 2011 Archaea nationallicence Bacteria nationallicence Diversity nationallicence Hot springs nationallicence Thaumarchaeota nationallicence Tibet nationallicence Huang Qiuyuan Department of Geology, Miami University, 45056, Oxford, OH, USA aut Dong Christina Badin High School, 571 Hamilton New London Road, 45013, Hamilton, OH, USA aut Dong Raymond Badin High School, 571 Hamilton New London Road, 45013, Hamilton, OH, USA aut Jiang Hongchen State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, 100083, Beijing, China aut Wang Shang State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, 100083, Beijing, China aut Wang Genhou School of Earth Sciences and Resources, China University of Geosciences, 100083, Beijing, China aut Fang Bin School of Water Resources and Environment, China University of Geosciences, 100083, Beijing, China aut Ding Xiaoxue School of Earth Sciences and Resources, China University of Geosciences, 100083, Beijing, China aut Niu Lu School of Earth Sciences and Resources, China University of Geosciences, 100083, Beijing, China aut Li Xin School of Earth Sciences and Resources, China University of Geosciences, 100083, Beijing, China aut Zhang Chuanlun State Key Laboratory of Marine Geology, Tongji University, 200092, Shanghai, China aut Dong Hailiang Department of Geology, Miami University, 45056, Oxford, OH, USA aut Extremophiles Springer Japan 15/5(2011-09-01), 549-563 1431-0651 15:5<549 2011 15 792 https://doi.org/10.1007/s00792-011-0386-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0386-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang Qiuyuan Department of Geology, Miami University, 45056, Oxford, OH, USA aut NATIONALLICENCE 700 1- Dong Christina Badin High School, 571 Hamilton New London Road, 45013, Hamilton, OH, USA aut NATIONALLICENCE 700 1- Dong Raymond Badin High School, 571 Hamilton New London Road, 45013, Hamilton, OH, USA aut NATIONALLICENCE 700 1- Jiang Hongchen State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, 100083, Beijing, China aut NATIONALLICENCE 700 1- Wang Shang State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, 100083, Beijing, China aut NATIONALLICENCE 700 1- Wang Genhou School of Earth Sciences and Resources, China University of Geosciences, 100083, Beijing, China aut NATIONALLICENCE 700 1- Fang Bin School of Water Resources and Environment, China University of Geosciences, 100083, Beijing, China aut NATIONALLICENCE 700 1- Ding Xiaoxue School of Earth Sciences and Resources, China University of Geosciences, 100083, Beijing, China aut NATIONALLICENCE 700 1- Niu Lu School of Earth Sciences and Resources, China University of Geosciences, 100083, Beijing, China aut NATIONALLICENCE 700 1- Li Xin School of Earth Sciences and Resources, China University of Geosciences, 100083, Beijing, China aut NATIONALLICENCE 700 1- Zhang Chuanlun State Key Laboratory of Marine Geology, Tongji University, 200092, Shanghai, China aut NATIONALLICENCE 700 1- Dong Hailiang Department of Geology, Miami University, 45056, Oxford, OH, USA aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/5(2011-09-01), 549-563 1431-0651 15:5<549 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer