caa a22 4500 445818689 CHVBK 20180317145237.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00792-011-0391-2 doi (NATIONALLICENCE)springer-10.1007/s00792-011-0391-2 Bioprocessing of agricultural residues to ethanol utilizing a cellulolytic extremophile [Elektronische Daten] [Vasudeo Zambare, Aditya Bhalla, Kasiviswanath Muthukumarappan, Rajesh Sani, Lew Christopher] A recently discovered thermophilic isolate, Geobacillus sp. R7, was shown to produce a thermostable cellulase with a high hydrolytic potential when grown on extrusion-pretreated agricultural residues such corn stover and prairie cord grass. At 70°C and 15-20% solids, the thermostable cellulase was able to partially liquefy solid biomass only after 36h of hydrolysis time. The hydrolytic capabilities of Geobacillus sp. R7 cellulase were comparable to those of a commercial cellulase. Fermentation of the enzymatic hydrolyzates with Saccharomyces cerevisiae ATCC 24860 produced ethanol yields of 0.45-0.50g ethanol/g glucose with more than 99% glucose utilization. It was further demonstrated that Geobacillus sp. R7 can ferment the lignocellulosic substrates to ethanol in a single step that could facilitate the development of a consolidated bioprocessing as an alternative approach for bioethanol production with outstanding potential for cost reductions. Springer, 2011 Bioprocessing nationallicence Agroresidues nationallicence Bioethanol nationallicence Cellulase nationallicence Geobacillus nationallicence Consolidated bioprocessing nationallicence High solids nationallicence Enzymatic hydrolysis nationallicence Zambare Vasudeo Center for Bioprocessing Research and Development, South Dakota School of Mines and Technology, 501 East Saint Joseph, 57701, Rapid City, SD, USA aut Bhalla Aditya Center for Bioprocessing Research and Development, South Dakota School of Mines and Technology, 501 East Saint Joseph, 57701, Rapid City, SD, USA aut Muthukumarappan Kasiviswanath Department of Agricultural and Biosystems Engineering, Center for Bioprocessing Research and Development, South Dakota State University, Brookings, SD, USA aut Sani Rajesh Center for Bioprocessing Research and Development, South Dakota School of Mines and Technology, 501 East Saint Joseph, 57701, Rapid City, SD, USA aut Christopher Lew Center for Bioprocessing Research and Development, South Dakota School of Mines and Technology, 501 East Saint Joseph, 57701, Rapid City, SD, USA aut Extremophiles Springer Japan 15/5(2011-09-01), 611-618 1431-0651 15:5<611 2011 15 792 https://doi.org/10.1007/s00792-011-0391-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00792-011-0391-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zambare Vasudeo Center for Bioprocessing Research and Development, South Dakota School of Mines and Technology, 501 East Saint Joseph, 57701, Rapid City, SD, USA aut NATIONALLICENCE 700 1- Bhalla Aditya Center for Bioprocessing Research and Development, South Dakota School of Mines and Technology, 501 East Saint Joseph, 57701, Rapid City, SD, USA aut NATIONALLICENCE 700 1- Muthukumarappan Kasiviswanath Department of Agricultural and Biosystems Engineering, Center for Bioprocessing Research and Development, South Dakota State University, Brookings, SD, USA aut NATIONALLICENCE 700 1- Sani Rajesh Center for Bioprocessing Research and Development, South Dakota School of Mines and Technology, 501 East Saint Joseph, 57701, Rapid City, SD, USA aut NATIONALLICENCE 700 1- Christopher Lew Center for Bioprocessing Research and Development, South Dakota School of Mines and Technology, 501 East Saint Joseph, 57701, Rapid City, SD, USA aut NATIONALLICENCE 773 0- Extremophiles Springer Japan 15/5(2011-09-01), 611-618 1431-0651 15:5<611 2011 15 792 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer